Glycated Plasma Proteins as More Sensitive Markers for Glycemic Control in Type 1 Diabetes
| Autor: | Qibin Zhang, Lina Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics medicine.medical_specialty Clinical Biochemistry Glycemic Control 03 medical and health sciences chemistry.chemical_compound Diabetes mellitus Internal medicine medicine Humans Glycemic Glycated Hemoglobin Type 1 diabetes 030102 biochemistry & molecular biology Receiver operating characteristic business.industry medicine.disease Blood proteins 030104 developmental biology Endocrinology Diabetes Mellitus Type 1 chemistry Proteome Female Glycated hemoglobin business Biomarkers Half-Life |
| Zdroj: | Proteomics. Clinical applications. 14(2) |
| ISSN: | 1862-8354 |
| Popis: | Purpose Glycated hemoglobin (HbA1c) is used clinically for diagnosis and therapeutic management of diabetes. However, HbA1c reflects average blood glucose level over a long period. The aim of this study is to look for short period, more sensitive protein markers that correlate better with glycemic level. Experimental design The glycated proteome of human plasma from type 1 diabetic individuals with good and poor (n = 20 each) glycemic control are analyzed using an online two-dimensional proteomics approach. Selected glycated peptides are further validated for their potential as candidate biomarkers using parallel reaction monitoring. Results 305 glycated peptides are quantified and 290 are significantly increased in samples with poor glycemic control. 76 of the 88 selected glycated peptides have receiver operating characteristic area under curve (AUC) values greater than 0.8. Six validated glycated peptides with high AUC show high correlation with HbA1c and have higher fold changes between poor and good glycemic control than HbA1c. The parent proteins have half-lives shorter than HbA1c. Conclusions and clinical relevance Using an advanced proteomics platform for protein glycation analysis, glycated peptides and proteins are identified that are promising as more sensitive, shorter term indicators of glycemic control in diabetic patients than the commonly used HbA1c. |
| Databáze: | OpenAIRE |
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