Desmoplastic small round cell tumour: Cytological and immunocytochemical features
| Autor: | Jeni Bortolan, Fernando Schmitt, Maria Dirlei Begnami, Nara de Matos Granja, Adhemar Longatto Filho |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2005 |
| Předmět: |
0303 health sciences
Pathology medicine.medical_specialty biology medicine.diagnostic_test Desmoplastic small-round-cell tumor business.industry Chromogranin A Papanicolaou stain Vimentin Case Report medicine.disease 3. Good health Pathology and Forensic Medicine 03 medical and health sciences Serous fluid 0302 clinical medicine Fine-needle aspiration 030220 oncology & carcinogenesis Biopsy biology.protein medicine Immunohistochemistry business 030304 developmental biology |
| Zdroj: | CytoJournal Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0974-5963 |
| Popis: | Background: Desmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive neoplasm. The cytological diagnosis of these tumors can be difficult because they show morphological features quite similar to other small round blue cells tumors. We described four cases of DSRCT with cytological sampling: one obtained by fine needle aspiration biopsy (FNAB) and three from serous effusions. The corresponding immunocytochemical panel was also reviewed. Methods: Papanicolaou stained samples from FNAB and effusions were morphologically described. Immunoreaction with WT1 antibody was performed in all cytological samples. An immunohistochemical panel including the following antibodies was performed in the corresponding biopsies: 34BE12, AE1/AE3, Chromogranin A, CK20, CK7, CK8, Desmin, EMA, NSE, Vimentin and WT1. Results: The smears showed high cellularity with minor size alteration. Nuclei were round to oval, some of them with inconspicuous nucleoli. Tumor cells are clustered, showing rosette-like feature. Tumor cells in effusions and FNA were positive to WT1 in 3 of 4 cytology specimens (2 out 3 effusions and one FNA). Immunohistochemical reactions for vimentin, NSE, AE1/AE3 and WT1 were positive in all cases in tissue sections. Conclusion: The use of an adjunct immunocytochemical panel coupled with the cytomorphological characteristics allows the diagnosis of DSRCT in cytological specimens. (undefined) |
| Databáze: | OpenAIRE |
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