Effect of Endostatin on Preventing Postoperative Progression of Distant Metastasis in a Murine Lung Cancer Model
| Autor: | He-Lan, Wang, Tao, Ning, Mei, Li, Ze-Jun, Lu, Xi, Yan, Qian, Peng, Na, Lei, Hui, Zhang, Feng, Luo |
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| Rok vydání: | 2011 |
| Předmět: |
0301 basic medicine
Cancer Research Lung Neoplasms Angiogenesis Inhibitors Enzyme-Linked Immunosorbent Assay Kaplan-Meier Estimate General Medicine Flow Cytometry Immunohistochemistry Endostatins Mice Inbred C57BL Carcinoma Lewis Lung Disease Models Animal Mice Random Allocation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis Disease Progression Secondary Prevention Animals Female Postoperative Period |
| Zdroj: | Tumori Journal. 97:787-793 |
| ISSN: | 2038-2529 0300-8916 |
| DOI: | 10.1177/030089161109700617 |
| Popis: | Aims and Background The relapse and metastasis of cancer remain a predominant cause of death after surgical removal of the primary tumor. There is a positive linkage between the postoperative upregulation of systemic angiogenic activity and distant tumor metastasis. In the present study, we established a spontaneous metastasis model and investigated whether antiangiogenic therapy using endostatin could prevent the progression of distant metastasis after removal of the primary tumor. Methods Female C57BL/6 mice were implanted subcutaneously with 1 × 106 Lewis lung cancer cells. Twenty days after implantation of the cancer cells, the primary tumor was removed and the mice were randomly divided into three groups. The NS group received normal saline, the L-ES group received 3 mg/kg endostatin, and the H-ES group received 20 mg/kg endostatin intravenously daily for 10 days. The effect of endostatin on lung metastases and the survival time of the mice were observed. Flow cytometry and immunohistochemistry were carried out to assess the angiogenic activity. The serum endostatin levels in peripheral blood were measured using an enzyme-linked immunosorbent assay. Results The mean number of metastatic pulmonary nodules and the mean net lung weight in the NS, L-ES and H-ES groups was 10.2, 2.8 and 4.0, and 0.55g, 0.31g and 0.36g, respectively. The difference between the NS group and the endostatin-treated groups was statistically significant (P Conclusions Antiangiogenic therapy with endostatin is effective in inhibiting the postoperative progression of distant metastasis. |
| Databáze: | OpenAIRE |
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