ELF-EMF exposure decreases the peroxidase catalytic efficiency in vitro
| Autor: | Marius Mihasan, Răzvan Caliga, Calin Maniu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Immunology and Microbiology QH301-705.5 fe3+ containing horseradish peroxidase (hrp) General Neuroscience Kinetics Biology General Biochemistry Genetics and Molecular Biology In vitro Emf exposure 03 medical and health sciences 030104 developmental biology kinetics catalytic efficiency biology.protein Biophysics extremely low frequency electromagnetic field (elf-emf) Biology (General) Catalytic efficiency General Agricultural and Biological Sciences Peroxidase |
| Zdroj: | Open Life Sciences, Vol 11, Iss 1, Pp 71-77 (2016) |
| ISSN: | 2391-5412 |
| DOI: | 10.1515/biol-2016-0009 |
| Popis: | The activity of purified Fe3+ containing horseradish peroxidase (HRP) was recorded while the enzyme was exposed to two low frequency pulsed electromagnetic fields (ELF-EMFs) with different frequencies: 50 Hz/2.7 mT and 100 Hz/5.5 mT. No statistically significant difference in the Michaelis constant (Km) values between the control enzyme and the enzyme exposed to any of the two ELF-EMF used could be detected (53 ± 11 μM vs 67 ± 10 μM vs 42 ± 11 μM respectively), indicating that upon short exposure times, there are no large structural alterations of the enzyme that affect the substrate affinity. A 50 Hz/2.7 mT EMF did however, cause a significant decrease in the maximum rate (Vmax) value (from 3.31 ± 0.17 μmoles/min to 1.6 ± 0.19 μmoles/min, p-1 to 0.32 ± 0.04 s-1, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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