Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A
| Autor: | Stefania Valenzano, Maria C. DeRosa, Maureen McKeague, Annalisa De Girolamo, McKenzie Smith, Michelangelo Pascale, Ranganathan Velu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ochratoxin A aptamer ochratoxin A mycotoxins biorecognition fluorescent assay biosensing aptasensor microscale thermophoresis Research groups Health Toxicology and Mutagenesis Aptamer Metal Nanoparticles lcsh:Medicine Nanotechnology Biosensing Techniques Biology Toxicology 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Mycotoxin Ochratoxin Microscale thermophoresis lcsh:R 010401 analytical chemistry food and beverages Aptamers Nucleotide Ochratoxins 0104 chemical sciences Solutions 030104 developmental biology chemistry Biochemistry Nucleic acid Gold Biosensor |
| Zdroj: | Toxins, Vol 8, Iss 11, p 336 (2016) Toxins; Volume 8; Issue 11; Pages: 336 Toxins Toxins (Basel) 8 (2016): 336. doi:10.3390/toxins8110336 info:cnr-pdr/source/autori:McKeague M.; Velu R.; De Girolamo A.; Valenzano S.; Pascale M.; Smith M.; Derosa M.C./titolo:Comparison of in-solution biorecognition properties of aptamers against ochratoxin A/doi:10.3390%2Ftoxins8110336/rivista:Toxins (Basel)/anno:2016/pagina_da:336/pagina_a:/intervallo_pagine:336/volume:8 |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins8110336 |
| Popis: | Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications. |
| Databáze: | OpenAIRE |
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