Exosomal miR-182 regulates the effect of RECK on gallbladder cancer
| Autor: | Yong-Lin Yu, Li-Jin Zhao, Hong Zheng, Jin-Jing Wang, Xiao-Rong Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Therapeutic target Normal tissue Normal gallbladder Apoptosis Exosomes GPI-Linked Proteins Exosome Metastasis 03 medical and health sciences 0302 clinical medicine Quantitative polymerase chain reaction Cell Movement Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness Gallbladder cancer Neoplasm Metastasis RECK Aged Cell Proliferation Chemistry Gastroenterology Gallbladder Epithelial Cells General Medicine Case Control Study Middle Aged medicine.disease Blot Gene Expression Regulation Neoplastic MicroRNAs Real-time polymerase chain reaction 030220 oncology & carcinogenesis miR-182 Cancer research 030211 gastroenterology & hepatology Female Gallbladder Neoplasms |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 2219-2840 1007-9327 |
| Popis: | Background As the most common biliary malignancy, gallbladder cancer (GC) is an elderly-biased disease. Although extensive studies have elucidated the molecular mechanism of microRNA 182 (miR-182) and reversion-inducing-cysteine-rich protein with kazal motifs (RECK) in various cancers, the specific role of exosomal miR-182 and RECK in GC remains poorly understood. Aim To explore the relationship between exosomal miR-182/RECK and metastasis of GC. Methods Paired GC and adjacent normal tissues were collected from 78 patients. Quantitative polymerase chain reaction was employed to detect miR-182 and exosomal miR-182 expression, and Western blotting was conducted to determine RECK expression. In addition, the effects of exosomal miR-182/RECK on the biological function of human GC cells were observed. Moreover, the double luciferase reporter gene assay was applied to validate the targeting relationship between miR-182 and RECK. Results Compared with normal gallbladder epithelial cells, miR-182 was highly expressed in GC cells, while RECK had low expression. Exosomal miR-182 could be absorbed and transferred by cells. Exosomal miR-182 inhibited RECK expression and promoted the migration and invasion of GC cells. Conclusion Exosomal miR-182 can significantly promote the migration and invasion of GC cells by inhibiting RECK; thus miR-182 can be used as a therapeutic target for GC. |
| Databáze: | OpenAIRE |
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