A comparative assessment of the roles of CD8 and CD2 in the functions of activated murine CD8+ T lymphocytes
| Autor: | Jörg Kupsch, Klaus Eichmann, Angelika Ehrfeld, Astrid Reimann, Bernhard Maier, Kai M. Saizawa, Birgitta Clinchy |
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| Rok vydání: | 1991 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytotoxicity Immunologic medicine.medical_treatment T cell CD8 Antigens Immunology CD2 Antigens Receptors Antigen T-Cell Fluorescent Antibody Technique Biology Lymphocyte Activation Transfection T-Lymphocytes Regulatory Cell Line Interferon-gamma Mice Antigens CD medicine Immunology and Allergy Cytotoxic T cell Animals Receptors Immunologic Receptor Mice Inbred BALB C T-cell receptor Antibodies Monoclonal Flow Cytometry Cell biology Mice Inbred C57BL CTL Cytokine medicine.anatomical_structure Antigens Surface Signal transduction CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Immunology letters. 30(2) |
| ISSN: | 0165-2478 |
| Popis: | Both CD8 and CD2 are T cell surface receptors involved in physical cell interaction and in transmembrane signalling. The present paper addresses their role in the induction of two different functions of the cloned murine cytotoxic T cell C196: target cell lysis and IFN-gamma production. These functions were induced in C196 either by stimulation with the specific stimulator/target cell P815 or, bypassing specific recognition, by the aCD3 hybridoma 145-2C11 or by solid phase aTCR antibodies. These responses were tested for their susceptibility to inhibition/enhancement by a panel of aCD8 and aCD2 mAb. In addition, CD8 deficient and CD8/CD2 double-deficient variants of C196 were transfected with the CD8 and CD2 genes and the resulting cell lines were analysed for their functional capacities. The following results were obtained: (i) CD8 is primarily important in the specific recognition process of activated CTL; (ii) transmembrane signalling of activated CTL through the TCR does not require CD8, nor is it sensitive to modification through CD8; (iii) CTL can nevertheless be directly activated through CD8; however, this is restricted to induction of cytotoxicity but does not result in IFN-gamma production; (iv) CD2 does not seem to be important in any of these responses. |
| Databáze: | OpenAIRE |
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