A new sensitive approach for spectrofluorimetric assay of Milnacipran and Amisulpride in real plasma and pharmaceutical preparations via complexation with Eosin Y dye
| Autor: | Walid E. Eltoukhi, Mostafa A. Marzouq, Ahmed I. Hassan, Noha N. Atia |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Milnacipran hydrochloride 02 engineering and technology 010402 general chemistry 01 natural sciences Dosage form Analytical Chemistry chemistry.chemical_compound Milnacipran medicine Humans Amisulpride Eosin Y Instrumentation Spectroscopy Detection limit Chromatography Quenching (fluorescence) 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Spectrometry Fluorescence chemistry Eosine Yellowish-(YS) Female 0210 nano-technology Blood Chemical Analysis medicine.drug |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 214 |
| ISSN: | 1873-3557 |
| Popis: | An accurate, economic, rapid, reliable spectrofluorimetric assay was developing for the assay of definite anti-depressant drugs namely Amisulpride and Milnacipran hydrochloride, in those pharmaceutical preparation and biological fluid. The suggested method was established on the detection of quenching process resulting from the action of AMS and MCN to the native fluorescent EosinY. A binary complex between selected medications and Eosin Y was established in acetate buffer (0.2 M) at pH 3.3 & 4.0 for AMS and MCN respectively. The relative fluorescence capacity was determined at λex = 301.8 nm and λem = 542.7 nm. The calibration graphs had been linear through extent from 0.02 to 0.3 and 0.1–1 μg mL−1, to both dugs respectively. Detection limits have been 0.0047 & 0.0188 μg mL−1 while quantitation limits have been 0.0141 & 0.0569 μg mL−1 to AMS & MCN respectively. Developed assay has been validated in agreement with ICH recommendations. Due to high sensitivity of the described assay, it allows the quantitation of anti-depressant drugs through biological fluid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect