Effects of bacterial direct-fed microbials on ruminal characteristics, methane emission, and milk fatty acid composition in cows fed high- or low-starch diets

Autor: Christelle Philippeau, Pierre Nozière, Diego P. Morgavi, Claudette Berger, A. Lettat, Cécile Martin, Mathieu Silberberg, Anne Ferlay
Přispěvatelé: Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Danisco France SAS, CIFRE Danisco SAS research fellowship, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA )
Rok vydání: 2017
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
cow
Latin square
teneur en amidon
méthane
Food science
dairy milk fatty acid
2. Zero hunger
chemistry.chemical_classification
acide gras
biology
Fatty Acids
food and beverages
Starch
04 agricultural and veterinary sciences
Milk
bacterial direct-fed microbial
vache
microflore bactérienne
Digestion
Female
Composition (visual arts)
Methane
Rumen
Propionibacterium
ruminal fermentation
dietetic foods
fermentation ruminale
03 medical and health sciences
Lactobacillus rhamnosus
Genetics
Animals
Lactation
Dry matter
aliment de régime
[ SDV ] Life Sciences [q-bio]
0402 animal and dairy science
Fatty acid
biology.organism_classification
040201 dairy & animal science
Diet
030104 developmental biology
chemistry
marsh gas
Fermentation
Propionate
Cattle
Animal Science and Zoology
fatty acid
Lactobacillus plantarum
Food Science
Zdroj: Journal of Dairy Science
Journal of Dairy Science, American Dairy Science Association, 2017, 100 (4), pp.2637-2650. ⟨10.3168/jds.2016-11663⟩
Journal of Dairy Science, American Dairy Science Association, 2017, 100 (4), pp.2637-2650. 〈10.3168/jds.2016-11663〉
ISSN: 0022-0302
DOI: 10.3168/jds.2016-11663
Popis: This study investigated the effects of bacterial direct fed microbials (DFM) on ruminal fermentation and microbial characteristics, methane (CH4) emission, diet digestibility, and milk fatty acid (FA) composition in dairy cows fed diets formulated to induce different ruminal volatile fatty acid (VFA) profiles. Eight ruminally cannulated dairy cows were divided into 2 groups based on parity, days in milk, milk production, and body weight. Cows in each group were fed either a high-starch (38%, HS) or a low-starch (2%, LS) diet in a 55:45 forage-to-concentrate ratio on a dry matter (DM) basis. For each diet, cows were randomly assigned to 1 of 4 treatments in a Latin square design of (1) control (CON); (2) Propionibacterium P63 (P63); (3) P63 plus Lactobacillus plantarum 115 (P63+Lp); (4) P63 plus Lactobacillus rhamnosus 32(P63+Lr). Strains of DFM were administered at 10(10) cfu/d. Methane emission (using the sulfur hexafluoride tracer technique), total-tract digestibility, dry matter intake, and milk production and composition were quantified in wk 3. Ruminal fermentation and microbial characteristics were measured in wk 4. Data were analyzed using the mixed procedure of SAS (SAS Institute Inc., Cary, NC). The 2 diets induced different ruminal VFA profiles, with a greater proportion of propionate at the expense of acetate and butyrate for the HS diet. Greater concentrations of total bacteria and selected bacterial species of methanogenic Archaea were reported for the HS diet, whereas the protozoa concentration in HS decreased. For both diets, bacterial DFM supplementation raised ruminal pH (+0.18 pH units, on average) compared with CON. Irrespective of diet, P63+Lp and P63+Lr increased ruminal cellulase activity (3.8-fold, on average) compared with CON, but this effect was not associated with variations in ruminal microbial numbers. Irrespective of diet, no effect of bacterial DFM on ruminal VFA was observed. For the LS diet, supplementing cows with P63+Lr tended to decrease CH4 emission (26.5%, on average, when expressed per kilogram of milk or 4% fat-corrected milk). Only P63 supplementation to cows fed the HS diet affected the concentration of some milk FA, such as cis isomers of 18:1 and intermediates of ruminal biohydrogenation of polyunsaturated FA. Overall, bacterial DFM could be useful to stabilize ruminal pH. Their effects on CH4 production mitigation and milk FA profile depended on DFM strain and diet and should be confirmed under a greater variation of dietary conditions.
Databáze: OpenAIRE
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