Complex Regulation of the Bacillus subtilis Aconitase Gene
| Autor: | Abraham L. Sonenshein, Kiyoshi Tachikawa, Manoja Ratnayake-Lecamwasam, Mark A. Strauch, Hyun-Jin Kim, Sam-In Kim |
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| Rok vydání: | 2003 |
| Předmět: |
Repressor
Bacillus subtilis Regulatory Sequences Nucleic Acid Biology medicine.disease_cause Microbiology Aconitase Bacterial Proteins medicine Gene Regulation Citrates Promoter Regions Genetic Molecular Biology Psychological repression Transcription factor Gene Aconitate Hydratase Mutation fungi Promoter Gene Expression Regulation Bacterial biology.organism_classification Culture Media DNA-Binding Proteins Repressor Proteins Biochemistry Cell Division Transcription Factors |
| Zdroj: | Journal of Bacteriology. 185:1672-1680 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.185.5.1672-1680.2003 |
| Popis: | The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase ( citB ) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive. |
| Databáze: | OpenAIRE |
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