Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Autor: Han Deng, Jianquan Wang, Shiqi Hu, Chengwan Xia, Yongfeng Chen, Jiongru Pan, Yuxin Wang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Near-Infrared Fluorescence Imaging
Surgical margin
Neoplasm
Residual
Arbitrary unit
030218 nuclear medicine & medical imaging
Mice
chemistry.chemical_compound
0302 clinical medicine
Surgical oncology
Coloring Agents
Spectroscopy
Near-Infrared
Optical Imaging
Margins of Excision
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Primary tumor
Indocyanine green
Surgery
Computer-Assisted
Oncology
030220 oncology & carcinogenesis
Female
Mouth Neoplasms
lcsh:Surgery
Near-infrared fluorescence imaging
Oral squamous cell cancer
lcsh:RC254-282
03 medical and health sciences
Cell Line
Tumor
Quantification
medicine
Animals
Humans
In patient
Basal cell
Aged
Squamous Cell Carcinoma of Head and Neck
business.industry
Research
Mouth Mucosa
lcsh:RD1-811
medicine.disease
Xenograft Model Antitumor Assays
chemistry
Surgery
Neoplasm Recurrence
Local
Nuclear medicine
business
Zdroj: World Journal of Surgical Oncology, Vol 18, Iss 1, Pp 1-8 (2020)
World Journal of Surgical Oncology
ISSN: 1477-7819
DOI: 10.1186/s12957-020-01874-z
Popis: Background Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperatively for OSCC. Methods Cytological and animal experiments were first performed to confirm the feasibility of monitoring surgical margins with NIF imaging and spectroscopy. Then, 20 patients with OSCC were included in the clinical trials. At 6–8 h after 0.75 mg/kg indocyanine green (ICG) injection, all patients underwent surgery with NIF imaging. During the surgery, both NIF images and quantified fluorescence intensity were acquired to monitor the surgical margins. Results In cytological and animal experiments, the results showed it was feasible to monitor surgical margins with NIF imaging and spectroscopy. Fluorescence was detected in primary tumors in all patients. The fluorescence intensities of the tumor, peritumoral, and normal tissues were 398.863 ± 151.47, 278.52 ± 84.89, and 274.5 ± 100.93 arbitrary units (AUs), respectively (P < 0.05). The SBR of tumor to peritumoral tissue and normal tissues was computed to be 1.45 ± 0.36 and 1.56 ± 0.41, respectively. After primary tumor excision, the wounds showed abnormal fluorescence in four patients (4/20), and residual cancer cells were confirmed by pathological examination in two patients (2/20). Conclusion These findings confirmed the complementary value of NIF imaging during radical tumor resection of OSCC. Before tumor resection, we could utilize the fluorescence margin produced by ICG NIF imaging to determine the surgical margin. Moreover, after tumor blocks were removed, the status of surgical margin could also be evaluated rapidly by ICG NIF imaging of tumor bed and in vitro specimens.
Databáze: OpenAIRE
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