Characterization of novel exopolysaccharide of Enterococcus faecium WEFA23 from infant and demonstration of its in vitro biological properties
| Autor: | Weijun He, Zhihong Zhang, Hua Wei, Kaiying Jia, Hui Zhan, Xueying Tao, Zheling Zeng, Zhengqi Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Enterococcus faecium Mannose 02 engineering and technology Biochemistry Bacterial Adhesion Feces 03 medical and health sciences chemistry.chemical_compound Picrates Structural Biology medicine Humans Food science Molecular Biology 030304 developmental biology 0303 health sciences biology Chemistry Biphenyl Compounds Monosaccharides Polysaccharides Bacterial Infant General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Listeria monocytogenes In vitro Molecular Weight Galactose Hydroxyl radical Fermentation 0210 nano-technology HT29 Cells |
| Zdroj: | International Journal of Biological Macromolecules. 128:710-717 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.12.245 |
| Popis: | In this study exopolysaccharide (EPS) of Enterococcus faecium WEFA23 from healthy infant's feces was yielded as high as 130 mg/L by fermentation. By purification the EPS was further fractioned into A23-1, A23-2, A23-3 and A23-4 on HiTrap Q HP and Superdex G-200 column. As the major purified fractions, A23-2 and A23-4 were analyzed for the preliminary structures and investigated for the biological properties in vitro. The molecular weight of A23-2 and A23-4 was 2.50 × 104 Da and 3.23 × 104 Da, respectively. A23-2 was composed of mannose, glucose and galactose with a ratio of 1.38:1.00:1.42, while A23-4 consisted of only mannose. The antioxidant ability of A23-2 was significantly higher than that of A23-4, as proved by scavenging test of DPPH radical, hydroxyl radical and superoxide radical. Both A23-2 and A23-4 exhibited strong inhibition against the adhesion of L. monocytogenes CMCC54007 on HT-29 cells no matter for the styles of competition, displacement and exclusion. Notably, for exclusion they had a maximal inhibition rate of 89.85 ± 0.46% and 90.81 ± 2.29%, respectively, at 200 μg/mL. |
| Databáze: | OpenAIRE |
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