Nucleotide sequence of the chicken HMGI-C cDNA and expression of the HMGI-C and IGF1 genes in autosomal dwarf chicken embryos
| Autor: | E. Limpens, J.J. van der Poel, C.P. Ruyter-Spira, Martien A. M. Groenen, J. Herbergs, T.A.Y. Ayoubi, J.A. Marsh |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system
DNA Complementary animal structures Molecular Sequence Data Biophysics Gene Expression Locus (genetics) Sequence alignment Chick Embryo Biology Biochemistry Structural Biology Complementary DNA Gene expression Genetics Animals Coding region Amino Acid Sequence RNA Messenger Cloning Molecular Insulin-Like Growth Factor I Gene Peptide sequence Base Sequence HMGA2 Protein High Mobility Group Proteins Nucleic acid sequence Molecular biology embryonic structures RNA Chickens Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1399:83-87 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(98)00101-8 |
| Popis: | Mutations in the genes for high mobility group protein I-C (HMGI-C) and insulin-like growth factor 1 (IGF1) are known to be responsible for dwarf phenotypes in the mouse. Because the locus for autosomal dwarfism (adw) in the chicken maps to a region which is syntenic to a region in the human and mouse in which the HMGI-C and IGF1 genes are located, HMGI-C and IGF1 are likely candidate genes for adw in the chicken. In this study their possible role in the establishment of this phenotype has been investigated. We have cloned and sequenced the complete coding region of the chicken HMGI-C cDNA. Comparison with its human counterpart revealed a nucleotide sequence conservation of 84%. Only nine amino acids are present principally in the N-terminal segment before the first DNA-binding domain. Northern blot analysis showed no difference in the expression of the HMGI-C gene between adw and wild-type chicken embryos. Also no mutations in either the HMGI-C or the IGF1 RNA nucleotide sequence were detected in adw chicken embryos. |
| Databáze: | OpenAIRE |
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