TRIM37 targets AKT in the growth of lung cancer cells
| Autor: | Chunluan Yuan, Haijun Sun, Chuanyong Mu, Xueqin Pang, Shumin Dong, Shiying Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
BCL2 OncoTargets and Therapy Viral vector 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA interference medicine Pharmacology (medical) LY294002 TRIM37 Lung cancer Protein kinase B Original Research biology Cell growth AKT apoptosis medicine.disease Ubiquitin ligase 030104 developmental biology cell proliferation Oncology chemistry Apoptosis BAX 030220 oncology & carcinogenesis biology.protein Cancer research |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| Popis: | Shumin Dong,1 Xueqin Pang,2 Haijun Sun,3 Chunluan Yuan,4 Chuanyong Mu,5 Shiying Zheng6 1Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, The First People’s Hospital of Lianyungang City, Lianyungang, Jiangsu 222000, People’s Republic of China; 2Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, People’s Republic of China; 3Department of Thoracic Surgery, The First People’s Hospital of Lianyungang City, Lianyungang, Jiangsu 222000, People’s Republic of China; 4Department of Oncology, The First People’s Hospital of Lianyungang City, Lianyungang, Jiangsu 222000, People’s Republic of China; 5Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, People’s Republic of China; 6Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, People’s Republic of China Background: TRIM37 is an ubiquitin E3 ligase. Growing evidence has demonstrated the high value of TRIM37 as a potential biomarker for diagnosis of certain cancers. However, the biological function of TRIM37 in lung cancer is still unknown.Materials and methods: In order to gain a deep insight into the function of TRIM37 in lung cancer cells, in the present study lentiviral vector was used to mediate RNA interference and overexpression of TRIM37 in lung cancer cells (H292, H358, and H1299). In addition, a specific AKT inhibitor LY294002 was utilized to examine the correlation between the expression of TRIM37 and AKT.Results: TRIM37 acts as a positive regulator of cell proliferation in lung cancer cells. Moreover, cell apoptosis analyses showed the antiapoptosis function of TRIM37, which was mainly dependent on the regulation of BCL2 and BAX. Our results also indicated that AKT might be a target of TRIM37 in lung cancer cells.Conclusion: This research not only helps in understanding the molecular mechanisms of TRIM37 in detail but also provides evidence to develop novel biomarkers for lung cancer diagnosis. Keywords: TRIM37, cell proliferation, apoptosis, BCL2, BAX, AKT |
| Databáze: | OpenAIRE |
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