Adenosine analogs inhibit the guanine-7-methylation of mRNA cap structures
| Autor: | Thomas O. Sitz, Guojun Bu, Keith A. Oxenrider |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
RNA Caps
Adenosine 3-Deazaneplanocin A Guanine Biophysics In Vitro Techniques Biology Methylation Biochemistry mRNA methylation Mice chemistry.chemical_compound Structural Biology Genetics medicine Animals Neplanocin A Ethionine Carcinoma Ehrlich Tumor Molecular Biology chemistry.chemical_classification Messenger RNA RNA Guanine-7-methyltransferase Methyltransferases Cell Biology S-Adenosylhomocysteine Cap Molecular biology Enzyme chemistry MRNA methylation medicine.drug |
| Zdroj: | FEBS Letters. 316:273-277 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)81307-l |
| Popis: | The adenosine analogs neplanocin A and deazaneplanocin A were observed to inhibit the in vivo guanine-7-methylation of mRNA cap structure using a new assay for hypomethylated RNA. Treatment of cultured mammalian cells with these adenosine analogs resulted in the same extent of hypomethylation of cap structure as did ethionine injection in mice. Neplanocin A and its non-metabolizable analog 3-deazaneplanocin A show the same maximal level of inhibition of methylation suggesting that these adenosine analogs exert their effects by elevating S-adenosylhomocysteine levels rather than by conversion to other inhibitory compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text