Gene expression associated with water-stress adaptation of rice cells and identification of two genes as hsp 70 and ubiquitin

Autor: Raimundo Villarroel, Marc Van Montagu, Chris Simoens, Chumpel Borkird
Rok vydání: 1991
Předmět:
Zdroj: Physiologia Plantarum. 82:449-457
ISSN: 1399-3054
0031-9317
DOI: 10.1034/j.1399-3054.1991.820318.x
Popis: We have isolated polyethylene glycol (PEG)-adapted rice cells that can proliferate in a medium with 20% PEG as well as in a medium with 1% NaCl. The adapted-cells overproduce a set of proteins whose roles may be associated with water-stress adaptation. To isolate genes encoding these proteins, we differentially screened a cDNA library and obtained 5 cDNA clones which showed preferential hybridization to mRNA of adapted cells. The present paper describes the pattern of expression and the sequence analysis of these 5 genes. Sequence analysis of partial cDNAs indicates that two genes encode the 70 kDa heat shock protein and the ubiquitin but the identities of the other 3 are not known. The expression of all 5 genes fluctuates slightly during the growth cycle of the PEG-adapted cells grown in the control or in the PEG-containing medium. In contrast, gene expression in the parental cells fluctuates to a much greater extent and always begins with an enhanced expression during the first day after subculture. Sub-lethal concentrations of PEG or NaCl have no immediate effect on gene expression in parental cells but NaCl may have a long term effect in enhancing the expression of two genes after 5 days. Abscisic acid (ABA) has no effect on the expression of 4 genes but suppresses the expression of one gene. The roles of these genes in water-stress adaptation of plant cells are discussed.
Databáze: OpenAIRE
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