Mismatch repair gene defects in sporadic colorectal cancer enhance immune surveillance
| Autor: | Melania Scarpa, Marco Scarpa, Nicolò Bassi, Carlo Castoro, Silvia Basato, Angelo Paolo Dei Tos, Alain Fiorot, Lucia Dallagnese, Cesare Ruffolo, Ignazio Castagliuolo, Anna Pozza, Fabio Canal, Francesca Erroi, Andrea Porzionato |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Oncology
Male medicine.medical_specialty Pathology congenital hereditary and neonatal diseases and abnormalities Colorectal cancer chemical and pharmacologic phenomena Kaplan-Meier Estimate MLH1 DNA Mismatch Repair Polymerase Chain Reaction Mismatch repair Lymphocytes Tumor-Infiltrating CD80 Surgical oncology Internal medicine medicine Humans neoplasms Survival analysis Aged Retrospective Studies Aged 80 and over Tissue microarray Immune surveillance business.industry Middle Aged medicine.disease Flow Cytometry Molecular medicine Immunohistochemistry digestive system diseases MSH6 MSH2 Tissue Array Analysis B7-1 Antigen Female Microsatellite Instability business Colorectal Neoplasms HT29 Cells Research Paper |
| Zdroj: | Oncotarget |
| Popis: | // Marco Scarpa 1,* , Cesare Ruffolo 2,* , Fabio Canal 3 , Melania Scarpa 1 , Silvia Basato 4 , Francesca Erroi 4 , Alain Fiorot 2 , Lucia Dall’Agnese 4 , Anna Pozza 4 , Andrea Porzionato 5 , Ignazio Castagliuolo 5 , Angelo P. Dei Tos 3 , Nicolo Bassi 2 and Carlo Castoro 1 1 Surgical Oncology Unit, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy 2 General Surgery Unit (IV), “Ca’ Foncello” Hospital, Treviso, Italy 3 Pathology Unit, “Ca’ Foncello” Hospital, Treviso, Italy 4 Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy 5 Department of Molecular Medicine, University of Padova, Padova, Italy * These authors have contributed equally to this work Correspondence to: Marco Scarpa, email: // Keywords : mismatch repair, colorectal cancer, immune surveillance, CD80 Received : June 03, 2015 Accepted : October 07, 2015 Published : October 19, 2015 Abstract Background: There is evidence that colorectal cancers (CRC) with DNA mismatch repair deficiency (MMR-D) are associated with a better prognosis than the generality of large bowel malignancies. Since an active immune surveillance process has been demonstrated to influence CRC outcome, we investigated whether MMR-D can enhance the immune response in CRC. Patients and Methods: A group of 113 consecutive patients operated for CRC (42 stage I or II and 71 with stage III or IV) was retrospectively analyzed. The expression of MMR genes (MSH2, MLH1, MSH6 and PSM2) and co-stimulatory molecule CD80 was assessed by tissue microarray immunohistochemistry. In addition, tumor infiltrating mononuclear cells (TIMC) and T cell subpopulations (CD4, CD8, T-bet and FoxP-3) were quantified. The effect of specific siRNA (siMSH2, siMLH1, siMSH6 and siPSM2) transfection in HT29 on CD80 expression was quantified by flow cytometry. Non parametric statistics and survival analysis were used. Results: Patients with MMR-D showed a higher T-bet/CD4 ratio ( p = 0.02), a higher rate of CD80 expression and CD8 lymphocyte infiltration compared to those with no MMR-D. Moreover, in the MMR-D group, the Treg marker FoxP-3 was not expressed ( p = 0.05). MMR-D patients with stage I or II and T-bet expression had a significant better survival ( p = 0.009). Silencing of MSH2, MLH1 and MSH6, but not PSM2, significantly increased the rate of CD80+ HT29 cells ( p = 0.007, p = 0.023 and p = 0.015, respectively). Conclusions: CRC with MMR-D showed a higher CD80 expression, and CD8+ and Th1 T-cell infiltration. In vitro silencing of MSH2, MLH1 and MSH6 significantly increased CD80+ cell rate. These results suggest an enhanced immune surveillance mechanism in presence of MMR-D. |
| Databáze: | OpenAIRE |
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