Nitric oxide synthases from photosynthetic organisms improve growth and confer nitrosative stress tolerance in E. coli. Insights on the pterin cofactor
| Autor: | Andrés Nejamkin, Natalia Correa-Aragunde, Del Castello F, Lorenzo Lamattina, Noelia Foresi |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Physiology Clinical Biochemistry Coenzymes Bacterial growth Nitric Oxide Biochemistry Cofactor Nitric oxide Ostreococcus tauri chemistry.chemical_compound Bacterial Proteins Chlorophyta Escherichia coli Pterin Synechococcus biology Algal Proteins biology.organism_classification Biopterin Recombinant Proteins Nitric oxide synthase Molecular Docking Simulation chemistry Nitrosative Stress biology.protein Nitric Oxide Synthase Reactive Oxygen Species Bacteria Protein Binding |
| Zdroj: | Nitric oxide : biology and chemistry. 119 |
| ISSN: | 1089-8611 |
| Popis: | Nitric oxide synthase (NOS) synthesizes NO from the substrate L-arginine (Arg). NOS with distinct biochemical properties were characterized from two photosynthetic microorganisms, the unicellular algae Ostreococcus tauri (OtNOS) and the cyanobacteria Synechococcus PCC 7335 (SyNOS). In this work we studied OtNOS and SyNOS recombinantly expressed in E. coli and analyzed bacterial growth and tolerance to nitrosative stress. Results show that the expression of OtNOS and SyNOS promotes bacterial growth and allows metabolizing Arg as N source. In accordance to a high NO producing activity, OtNOS expression induces the hmp flavohemoglobin in E. coli, suggesting that this strain is sensing nitrosative stress. The addition of 1 mM of the NO donor sodium nitroprusside (SNP) is toxic and generates a strong nitrosative stress. The expression of OtNOS or SyNOS reduced SNP toxicity restoring bacterial growth. Finally, using bioinformatic tools and ligand docking analyses, we propose tetrahydromonapterin (MH4), an endogenous pterin found in E. coli, as potential cofactor required for NOS catalytic activity. Our findings could be useful for the development of biotechnological applications using NOS expression to improve growth in bacteria.Key points- The NO synthase (NOS) from photosynthetic microorganisms were expressed in E. coli- Expression of NOS increases bacterial growth and tolerance to nitrosative stress.- Ligand docking analyses indicate tetrahydromonapterin (MH4) as potential NOS cofactor in E. coli. |
| Databáze: | OpenAIRE |
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