Chitosan and Kappa-Carrageenan Vaginal Acyclovir Formulations for Prevention of Genital Herpes. In Vitro and Ex Vivo Evaluation
| Autor: | Paulina Bermejo, Roberto Ruiz-Caro, María-Dolores Veiga, Araceli Martín-Illana, Rubén Carro, Luis-Miguel Bedoya, María-Pilar Sánchez-Sánchez, Aitana Tamayo, Anxo Fernández-Ferreiro, Francisco J. Otero-Espinar, M. J. Abad, Juan Rubio |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Farmacia e Tecnoloxía Farmacéutica, Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Sexual transmission
Cell Survival Farmacología Bioadhesive Vaginal mucoadhesive formulations Cytotoxicity Acyclovir Pharmaceutical Science ex vivo bioadhesion Carrageenan Kappa-carrageenan Article Cell Line Microbiology Chitosan kappa-carrageenan chemistry.chemical_compound swelling behaviour Drug Discovery medicine Ex vivo bioadhesion Humans lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) vaginal mucoadhesive formulations Acyclovir controlled release acyclovir controlled release Herpes Genitalis Biología celular Chemistry Vaginal delivery Controlled release Genital herpes lcsh:Biology (General) genital herpes cytotoxicity chitosan Swelling medicine.symptom Ex vivo Swelling behaviour |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Marine Drugs, Vol 13, Iss 9, Pp 5976-5992 (2015) E-Prints Complutense. Archivo Institucional de la UCM Digital.CSIC. Repositorio Institucional del CSIC Marine Drugs Volume 13 Issue 9 Pages 5976-5992 Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) |
| Popis: | Vaginal formulations for the prevention of sexually transmitted infections are currently gaining importance in drug development. Polysaccharides, such as chitosan and carrageenan, which have good binding capacity with mucosal tissues, are now included in vaginal delivery systems. Marine polymer-based vaginal mucoadhesive solid formulations have been developed for the controlled release of acyclovir, which may prevent the sexual transmission of the herpes simplex virus. Drug release studies were carried out in two media: simulated vaginal fluid and simulated vaginal fluid/simulated seminal fluid mixture. The bioadhesive capacity and permanence time of the bioadhesion, the prepared compacts, and compacted granules were determined ex vivo using bovine vaginal mucosa as substrate. Swelling processes were quantified to confirm the release data. Biocompatibility was evaluated through in vitro cellular toxicity assays, and the results showed that acyclovir and the rest of the materials had no cytotoxicity at the maximum concentration tested. The mixture of hydroxyl-propyl-methyl-cellulose with chitosan- or kappa-carrageenan-originated mucoadhesive systems that presented a complete and sustained release of acyclovir for a period of 8-9 days in both media. Swelling data revealed the formation of optimal mixed chitosan/hydroxyl-propyl-methyl-cellulose gels which could be appropriated for the prevention of sexual transmission of HSV. © 2015 by the authors; licensee MDPI, Basel, Switzerland. This work was supported by the Spanish Ministry of Economy and Competitiveness (MAT2012-34552). We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). |
| Databáze: | OpenAIRE |
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