A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine
| Autor: | Folkert Kuipers, Jelske N. van der Veen, Hanneke Vermeulen, Heleen de Vogel-van den Bosch, Sander M. Houten, Nicole de Wit, Roelof van der Meer, Michael Müller, Guido J. E. J. Hooiveld |
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| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM), Paediatric Metabolic Diseases, Laboratory Genetic Metabolic Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Physiology Receptors Cytoplasmic and Nuclear ATP-binding cassette transporter ACTIVATION Voeding Metabolisme en Genomica chemistry.chemical_compound Feces Intestine Small Npc1l1 ABSORPTION ATP Binding Cassette Transporter Subfamily G Member 5 Liver X Receptors Oligonucleotide Array Sequence Analysis Human Nutrition & Health Mice Knockout EXCRETION Reverse Transcriptase Polymerase Chain Reaction Reverse cholesterol transport Humane Voeding & Gezondheid Gastroenterology cellular cholesterol Orphan Nuclear Receptors Metabolism and Genomics secretion DNA-Binding Proteins medicine.anatomical_structure Cholesterol ABC transporters Metabolisme en Genomica SECRETION excretion Nutrition Metabolism and Genomics lipids (amino acids peptides and proteins) liver-x-receptor ATP Binding Cassette Transporter 1 medicine.medical_specialty mice Lipoproteins Biology METABOLISM Cholesterol 7 alpha-hydroxylase Models Biological fatty acids Bile Acids and Salts Voeding Physiology (medical) Internal medicine medicine Animals CELLULAR CHOLESTEROL Liver X receptor Triglycerides VLAG Nutrition cholesterol absorption LXR-ALPHA caco-2 cells Hepatology ATP Binding Cassette Transporter Subfamily G Member 8 Membrane Transport Proteins Epithelial Cells Dietary Fats Epithelium Small intestine Hydroxycholesterols Mice Inbred C57BL MICE Endocrinology chemistry Gene Expression Regulation Intestinal Absorption Caco-2 lxr-alpha CACO-2 CELLS activation ATP-Binding Cassette Transporters Hydroxymethylglutaryl CoA Reductases LIVER-X-RECEPTOR absorption metabolism |
| Zdroj: | ResearcherID American Journal of Physiology. Gastrointestinal and Liver Physiology, 294(5), G1171-G1180 American Journal of Physiology-Gastrointestinal and Liver Physiology, 294(5), G1171-G1180. AMER PHYSIOLOGICAL SOC American journal of physiology. Gastrointestinal and liver physiology, 294(5), G1171-G1180. American Physiological Society American Journal of Physiology. Gastrointestinal and Liver Physiology 294 (2008) 5 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine. Am J Physiol Gastrointest Liver Physiol 294: G1171-G1180, 2008. First published March 20, 2008; doi:10.1152/ajpgi.00360.2007.-Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 ( Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all down-regulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% ( P |
| Databáze: | OpenAIRE |
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