Talin folding as the tuning fork of cellular mechanotransduction
| Autor: | Rafael Tapia-Rojo, Alvaro Alonso-Caballero, Julio M. Fernandez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Talin
0301 basic medicine Protein Folding Magnetic tweezers Stochastic resonance Context (language use) 02 engineering and technology Mechanotransduction Cellular law.invention 03 medical and health sciences Protein Domains law Tweezers Mechanotransduction Tuning fork Physics Multidisciplinary Noise (signal processing) Dynamics (mechanics) Biological Sciences 021001 nanoscience & nanotechnology Single Molecule Imaging Folding (chemistry) 030104 developmental biology 0210 nano-technology Biological system |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2004091117 |
| Popis: | Cells continually sample their mechanical environment using exquisite force sensors such as talin, whose folding status triggers mechanotransduction pathways by recruiting binding partners. Mechanical signals in biology change quickly over time and are often embedded in noise; however, the mechanics of force-sensing proteins have only been tested using simple force protocols, such as constant or ramped forces. Here, using our magnetic tape head tweezers design, we measure the folding dynamics of single talin proteins in response to external mechanical noise and cyclic force perturbations. Our experiments demonstrate that talin filters out external mechanical noise but detects periodic force signals over a finely-tuned frequency range. Hence, talin operates as a mechanical bandpass filter, able to read and interpret frequency-dependent mechanical information through its folding dynamics. We describe our observations in the context of stochastic resonance, which we propose as a mechanism by which mechanosensing proteins could respond accurately to force signals in the naturally noisy biological environment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|