In vitro assessment of plant lectins with anti-pinwood nematode activity
Autor: | Zhu Fayin, Mao Shiqing, Qi Gaofu, Zhao Xiu-yun, Yu Zhiniu |
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Rok vydání: | 2008 |
Předmět: |
Nematoda
Pinellia ternata Pinellia Radiata Population Microbiology Agglutinin Botany Animals Nematode Infections education Ecology Evolution Behavior and Systematics education.field_of_study biology Liliaceae Antinematodal Agents Lectin Hemagglutination Tests biology.organism_classification Immunity Innate Lycoris radiata Nematode Lycoris biology.protein Rabbits Plant Lectins |
Zdroj: | Journal of Invertebrate Pathology. 98:40-45 |
ISSN: | 0022-2011 |
DOI: | 10.1016/j.jip.2007.11.004 |
Popis: | Two lectin proteins were purified from the corms of Pinellia ternata and Lycoris radiata. Both P. ternata agglutinin (PTA) protein and L. radiata agglutinin (LRA) protein formed polymers and coagulated both rabbit red blood cells and yeast cells. The two proteins were each diluted to different concentration and then mixed with pinewood nematodes, and nematode survival was measured. Results showed that the two lectin proteins showed significant levels of resistance against nematodes and the nematode population was significantly reduced, compared to PBS buffer without protein control group. The mean number of nematodes of two lectin proteins group was significantly lower than that of control group constantly throughout the assay period with differences being very significant at P |
Databáze: | OpenAIRE |
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