Soluble recombinant human metapneumovirus G protein is immunogenic but not protective
| Autor: | Joyce E. Johnson, Amy B. Podsiad, Sharon J. Tollefson, John V. Williams, Alex B. Ryder |
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| Rok vydání: | 2010 |
| Předmět: |
Glycosylation
Paramyxoviridae viruses Guinea Pigs Antibodies Viral Article Viral Proteins Affinity chromatography Antigen Human metapneumovirus Animals Metapneumovirus Mononegavirales Lung Glycoproteins Paramyxoviridae Infections General Veterinary General Immunology and Microbiology biology Immunogenicity Vaccination Public Health Environmental and Occupational Health virus diseases biology.organism_classification Antibodies Neutralizing Virology Recombinant Proteins Rats respiratory tract diseases Infectious Diseases biology.protein Cytokines Molecular Medicine Antibody |
| Zdroj: | Vaccine. 28:4145-4152 |
| ISSN: | 0264-410X |
| Popis: | Human metapneumovirus (HMPV) expresses the major surface glycoproteins F and G. We evaluated the protective efficacy of immunization with G. We generated a recombinant form of G ectodomain (GDeltaTM) that was secreted from mammalian cells and purified by affinity chromatography. We tested the immunogenicity of GDeltaTM in cotton rats. Animals were immunized with PBS, GDeltaTM alone or adjuvanted, or were infected once with HMPV, and challenged with live HMPV at 28 days. Animals vaccinated with adjuvanted and non-adjuvanted GDeltaTM developed high levels of serum antibodies to both recombinant and native G protein; however, vaccinated animals did not develop neutralizing antibodies and were not protected against virus challenge. Unlike the analogous non-fusion glycoproteins of other human paramyxoviruses, HMPV G does not appear to be a protective antigen. This represents an unusual feature of HMPV. |
| Databáze: | OpenAIRE |
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