Rapid vaccination using an acetalated dextran microparticulate subunit vaccine confers protection against triplicate challenge by bacillus anthracis
| Autor: | Eric M. Bachelder, Kevin J. Peine, Margret A. Elberson, Kristy M. Ainslie, Angela M. Prouty, Mariko. E. Fonseca, Sadhana Sharma, Matthew D. Gallovic, Andrea M. Keane-Myers, John T. Pesce, Matthew G. Bell, Hassan Borteh, Kevin L. Schully |
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| Rok vydání: | 2012 |
| Předmět: |
Bacterial Toxins
Pharmaceutical Science Anthrax Vaccines medicine.disease_cause Neutralization Microbiology Anthrax chemistry.chemical_compound Mice Immune system Medicine Animals Pharmacology (medical) Pharmacology Antigens Bacterial Drug Carriers biology business.industry Toxin Organic Chemistry Toll-Like Receptors Vaccination Imidazoles Acetylation Dextrans biology.organism_classification Virology Bacillus anthracis Bacterial vaccine chemistry Antibody Formation Vaccines Subunit biology.protein Molecular Medicine Antibody Resiquimod business Biotechnology |
| Zdroj: | Pharmaceutical research. 30(5) |
| ISSN: | 1573-904X |
| Popis: | A rapid immune response is required to prevent death from Anthrax, caused by Bacillus anthracis. We formulated a vaccine carrier comprised of acetalated dextran microparticles encapsulating recombinant protective antigen (rPA) and resiquimod (a toll-like receptor 7/8 agonist). We were able to protect against triplicate lethal challenge by vaccinating twice (Days 0, 7) and then aggressively challenging on Days 14, 21, 28. A significantly higher level of antibodies was generated by day 14 with the encapsulated group compared to the conventional rPA and alum group. Antibodies produced by the co-encapsulated group were only weakly-neutralizing in toxin neutralization; however, survival was not dependent on toxin neutralization, as all vaccine formulations survived all challenges except control groups. Post-mortem culture swabs taken from the hearts of vaccinated groups that did not produce significant neutralizing titers failed to grow B. anthracis. Results indicate that protective antibodies are not required for rapid protection; indeed, cytokine results indicate that T cell protection may play a role in protection from anthrax. We report the first instance of use of a particulate carrier to generate a rapid protective immunity against anthrax. |
| Databáze: | OpenAIRE |
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