Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
| Autor: | Maurilio J. Soares, Carla V. de Paula Lima, Stenio Perdigão Fragoso, Rosana Elisa Gonçalves Gonçalves Pinho, Ligia Cristina Kalb Souza |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Angomonas deanei lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Trypanosoma cruzi Blotting Western lcsh:QR1-502 Protozoan Proteins Fluorescent Antibody Technique trypanosomatids Trypanosoma brucei confocal microscopy lcsh:Microbiology Mice parasitic diseases Euglenozoa Animals Trypanosomatina Leishmania major Crithidia fasciculata Microscopy Confocal biology fungi Articles biology.organism_classification Molecular biology Actins Cytoplasm Electrophoresis Polyacrylamide Gel actin |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 5, Pages: 631-636, Published: AUG 2013 Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 5, Pp 631-636 (2013) |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/0074-0276108052013015 |
| Popis: | Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids. |
| Databáze: | OpenAIRE |
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