Proteomic Analysis Reveals that Topoisomerase 2A is Associated with Defective Sperm Head Morphology
| Autor: | Ana Izabel Silva Balbin Villaverde, Jacob Netherton, Mark Baker, Rachel A Ogle, Louise Hetherington, Hubert Hondermarck |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Proteomics Sperm Head Motility Morphology (biology) Biochemistry Analytical Chemistry Male infertility Andrology 03 medical and health sciences Tandem Mass Spectrometry medicine Humans Molecular Biology Infertility Male Aged 030304 developmental biology 0303 health sciences biology business.industry Research Topoisomerase 030302 biochemistry & molecular biology Middle Aged medicine.disease Sperm DNA Topoisomerases Type II biology.protein Etiology Biomarker (medicine) business Biomarkers Chromatography Liquid |
| Zdroj: | Mol Cell Proteomics |
| ISSN: | 1535-9476 |
| Popis: | Male infertility is widespread and estimated to affect 1 in 20 men. Although in some cases the etiology of the condition is well understood, for at least 50% of men, the underlying cause is yet to be classified. Male infertility, or subfertility, is often diagnosed by looking at total sperm produced, motility of the cells and overall morphology. Although counting spermatozoa and their associated motility is routine, morphology assessment is highly subjective, mainly because of the procedure being based on microscopic examination. A failure to diagnose male-infertility or sub-fertility has led to a situation where assisted conception is often used unnecessarily. As such, biomarkers of male infertility are needed to help establish a more consistent diagnosis. In the present study, we compared nuclear extracts from both high- and low-quality spermatozoa by LC-MS/MS based proteomic analysis. Our data shows that nuclear retention of specific proteins is a common facet among low-quality sperm cells. We demonstrate that the presence of Topoisomerase 2A in the sperm head is highly correlated to poor head morphology. Topoisomerase 2A is therefore a potential new biomarker for confirming male infertility in clinical practice. |
| Databáze: | OpenAIRE |
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