New Mass-Spectrometry-Compatible Degradable Surfactant for Tissue Proteomics

Autor:	Deyang Yu, Zachery R. Gregorich, Huseyin Guner, Ying Ge, Ying Hua Chang, Albert J. Chen, Leekyoung Hwang, Jianyi Zhang
Rok vydání:	2015
Předmět:	Proteomics Chromatography Swine General Chemistry Mass spectrometry Biochemistry Mass Spectrometry Article Surface-Active Agents chemistry.chemical_compound Membrane protein chemistry Pulmonary surfactant Protein purification Animals Biomarker discovery Sodium dodecyl sulfate Thermostability
Zdroj:	Journal of Proteome Research. 14:1587-1599
ISSN:	1535-3907 1535-3893
Popis:	Tissue proteomics is increasingly recognized for its role in biomarker discovery and disease mechanism investigation. However, protein solubility remains a significant challenge in mass spectrometry (MS)-based tissue proteomics. Conventional surfactants such as sodium dodecyl sulfate (SDS), the preferred surfactant for protein solubilization, are not compatible with MS. Herein, we have screened a library of surfactant-like compounds and discovered an MS-compatible degradable surfactant (MaSDeS) for tissue proteomics that solubilizes all categories of proteins with performance comparable to SDS. The use of MaSDeS in the tissue extraction significantly improves the total number of protein identifications from commonly used tissues, including tissue from the heart, liver, and lung. Notably, MaSDeS significantly enriches membrane proteins, which are often under-represented in proteomics studies. The acid degradable nature of MaSDeS makes it amenable for high-throughput mass spectrometry-based proteomics. In addition, the thermostability of MaSDeS allows for its use in experiments requiring high temperature to facilitate protein extraction and solubilization. Furthermore, we have shown that MaSDeS outperforms the other MS-compatible surfactants in terms of overall protein solubility and the total number of identified proteins in tissue proteomics. Thus, the use of MaSDeS will greatly advance tissue proteomics and realize its potential in basic biomedical and clinical research. MaSDeS could be utilized in a variety of proteomics studies as well as general biochemical and biological experiments that employ surfactants for protein solubilization.
Databáze:	OpenAIRE
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