beta-MHC transgene expression in suspended and mechanically overloaded/suspended soleus muscle of transgenic mice
| Autor: | G. L. Tsika, L. Gao, Richard W. Tsika, A. M. Fox, J. J. McCarthy |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty Myosin light-chain kinase Transcription Genetic Physiology Transgene Mice Transgenic Hindlimb Myosins Biology Mice Physiology (medical) Internal medicine Gene expression Myosin medicine Animals RNA Messenger Transgenes Northern blot Muscle Skeletal Promoter Regions Genetic Soleus muscle Myosin Heavy Chains Body Weight Organ Size Endocrinology Gene Expression Regulation Stress Mechanical |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 272:R1552-R1561 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.1997.272.5.r1552 |
| Popis: | Non-weight-bearing (NWB) activity [space flight and hindlimb suspension (HS)] results in the loss of soleus muscle mass, a slow-to-fast fiber-type conversion, and decreased beta-myosin heavy chain (beta-MHC) protein and mRNA expression. To identify beta-MHC promoter sequences required for decreased beta-MHC expression in response to HS, we have modified an existing noninvasive hindlimb unweighting model to accommodate the use of (transgenic) mice. After 2 wk of HS, body and muscle (soleus > gastrocnemius > plantaris) weights were decreased as was the proportion of histochemically classified type I fibers in HS soleus muscle. Northern blot analysis revealed decreases in endogenous mRNA representing beta-MHC, slow myosin light chain 1 and 2, and cardiac/slow troponin C, whereas those representing skeletal troponin C, muscle creatine kinase, and glyceraldehyde-3-phosphate dehydrogenase increased. Protein extracts prepared from HS soleus (SS) muscle of mice harboring transgenes comprised of 5.6 or 0.6 kilobase of wild type (wt) mouse beta-MHC promoter (beta 5.6 wt, beta 0.6wt) and those carrying the simultaneous mutation (mut) of the MCAT, C-rich, and beta e3 subregions (beta 5.6mut3, beta 0.6mut3) revealed decreases in chloramphenicol acetyltransferase (CAT) specific activity relative to respective controls. Decreased CAT mRNA was observed for transgene beta 5.6mut3, line 85. Two weeks of the simultaneous imposition of mechanical overload (synergist ablation) and HS (MOV/HS) countermanded the loss in absolute and normalized SS weight but did not decrease beta 0.6wt transgene expression. These transgenic results demonstrate that regulatory sequences within a 600-base pair beta-MHC promoter are sufficient to direct decreased transcription of beta-MHC transgenes after 2 wk of HS. |
| Databáze: | OpenAIRE |
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