Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress and vascular behavior
| Autor: | Raquel Cristine Silva Barcelos, Victor De Mello Palma, Cristiane Cademartori Danesi, Roberto Marinho Maciel, Marilise Escobar Burger, Luisa Machado Barin, Luciana Taschetto Vey, Karla Zanini Kantorski, Fernanda Maia Pillusky |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Light Thiobarbituric acid medicine.medical_treatment Biophysics Gingiva Photodynamic therapy Ascorbic Acid Pharmacology medicine.disease_cause Root Planing Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Scaling and root planing Anti-Infective Agents medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Periodontitis Radiation Photosensitizing Agents Radiological and Ultrasound Technology Vitamin C 030206 dentistry Glutathione medicine.disease Rats Methylene Blue Oxidative Stress 030104 developmental biology Biochemistry chemistry Photochemotherapy Blood Vessels Dental Scaling Oxidative stress |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 173 |
| ISSN: | 1873-2682 |
| Popis: | Background To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. Methods Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP + aPDT + MB dissolved in water (aPDT I); SRP + aPDT + MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30 days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. Results PC showed higher thiobarbituric acid reactive substances levels in 7/15/30 days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. Conclusion In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment. |
| Databáze: | OpenAIRE |
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