Structural and Physicochemical Characterization of Chitosan Obtained by UAE and its Effect on the Growth Inhibition of Pythium ultimum
| Autor: | Nelly Medina-Torres, Héctor Martín-López, Emanuel Herrera-Pool, Hugo Espinosa-Andrews, Stéphane Trombotto, Soledad Cecilia Pech-Cohuo, Ana Ramos-Díaz, Juan Carlos Cuevas-Bernardino, Neith Pacheco, Teresa Ayora-Talavera |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
deacetylation
Size-exclusion chromatography Litopenaeus vannamei 02 engineering and technology Plant Science macromolecular substances engineering.material 01 natural sciences Chitosan chemistry.chemical_compound Chitin Fourier transform infrared spectroscopy Solubility lcsh:Agriculture (General) antimicrobial activity biology 010405 organic chemistry Extraction (chemistry) technology industry and agriculture Pythium ultimum 021001 nanoscience & nanotechnology biology.organism_classification lcsh:S1-972 0104 chemical sciences carbohydrates (lipids) chemistry engineering Biopolymer chitosan 0210 nano-technology Agronomy and Crop Science ultrasound-assisted extraction Food Science Nuclear chemistry |
| Zdroj: | Agriculture Volume 10 Issue 10 Agriculture, Vol 10, Iss 464, p 464 (2020) |
| ISSN: | 2077-0472 |
| DOI: | 10.3390/agriculture10100464 |
| Popis: | The objective of this work was the recovery of chitosan by ultrasound-assisted extraction (UAE) from white shrimp (Litopenaeus vannamei) chitin, and the physicochemical and structural characterization of the obtained biopolymer, as well as its antimicrobial effect on Pythium ultimum growth. A 23 factorial design was used to evaluate chitosan extraction conditions. Instrumental analysis techniques for chitosan characterization and radial growth inhibition, as an antifungal activity test, were performed. The ultrasonically extracted chitosan (UC) reached a yield of 86.96% with 100% solubility, a degree of deacetylation (DDA) > 78%, molecular weight (MW) of 3.928 × 105 g mol&minus 1, and a crystallinity index (Icr) of 87%, calculated through nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy (FTIR), size exclusion chromatography (SEC), and X-ray diffraction (XRD), respectively. The inhibitory activity of the chitosan was evaluated against the oomycete Pythium ultimum, observing a 93% radial inhibition over 24 h. UAE proved to be an excellent alternative to the conventional deacetylation, reducing reaction time and obtaining a UC with higher MW and (Icr) than the commercial one, which could potentiate its applications. |
| Databáze: | OpenAIRE |
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