Experimental oral and ocularEncephalitozoon cuniculiinfection in rabbits
| Autor: | Edita Jeklova, Misa Skoric, K. Kovarcik, Jarmila Maskova, M. Faldyna, Vladimir Kummer, Matiasovic J, L. Leva |
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| Rok vydání: | 2010 |
| Předmět: |
Eye Infections
Antibodies Protozoan Lymphocyte proliferation Lymphocyte Activation Microsporidiosis Serology Immune system Immunity Animals Outbred Strains medicine Animals Encephalitozoon cuniculi Immunity Cellular biology fungi medicine.disease biology.organism_classification Encephalitozoonosis Disease Models Animal Infectious Diseases Immunoglobulin M Immunoglobulin G Antibody Formation Immunology biology.protein Animal Science and Zoology Parasitology Rabbits Antibody Mouth Diseases |
| Zdroj: | Parasitology. 137:1749-1757 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182010000648 |
| Popis: | SUMMARYEncephalitozoon cuniculiis an obligate intracellular pathogen that has a wide host distribution, but primarily affects rabbits. The aim of this study was to characterize both the cell-mediated and the antibody response in rabbits after experimental infection using 2 different infection routes: oral and ocular. SPF rabbits were infected with low (103spores) and high (107spores) infection doses. Monitored parameters included clinical signs, detection of spores in urine, antibody response detected with ELISA, and cell-mediated immunity detected by antigen-driven lymphocyte proliferation. At week 13 post-infection, half of the rabbits in each group were suppressed by intramuscular administration of dexamethasone. At week 18 post-infection, animals were euthanized. Clinical signs were mild with exacerbation after immunosuppression. Spores in urine and antigen-specific cell-mediated immunity were detected from weeks 5 and 4 post-infection, respectively. Specific IgM was detected 1 week after infection, and IgG antibodies followed 1 week later in rabbits infected with the high dose. Immunological responses were dose dependent. The authors can conclude that both oral and ocular experimental infection withE. cuniculiresulted in an immune response of the infected animals. Rabbits could be used as an experimental model for the study of ocular microsporidiosis. |
| Databáze: | OpenAIRE |
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