Uptake of radiolabeled 3,3',4,4'-tetrachlorobiphenyl into Japanese quail egg compartments and embryo following air cell and albumen injection
| Autor: | Karen M. Dean, Allegra M. Marcell, Mary Ann Ottinger, Meredith Bohannon, Leah D. Baltos, Andrew N. Iwaniuk |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty food.ingredient Embryo Nonmammalian Health Toxicology and Mutagenesis Embryonic Development Coturnix 010501 environmental sciences 01 natural sciences Chorioallantoic Membrane Andrology 03 medical and health sciences chemistry.chemical_compound Egg Shell food biology.animal Internal medicine Yolk medicine Environmental Chemistry Animals Incubation 0105 earth and related environmental sciences Ovum chemistry.chemical_classification biology Embryogenesis Fatty acid Embryo Embryonic stem cell Egg Yolk Polychlorinated Biphenyls Quail 030104 developmental biology Endocrinology chemistry embryonic structures Radiopharmaceuticals Xenobiotic |
| Zdroj: | Environmental toxicology and chemistry. 37(1) |
| ISSN: | 1552-8618 |
| Popis: | The avian embryo is an excellent model for testing adverse developmental effects of environmental chemicals as well as uptake and movement of xenobiotics within the egg compartments. Before incubation at embryonic day 0, 14 C 3,3',4,4'-tetrachlorobiphenyl (14 C PCB 77) was injected into Japanese quail eggs either onto the air cell or into the albumen. All egg components were collected on embryonic day 1, 5, or 10, and concentrations of 14 C PCB 77 were measured in various egg components (shell, membrane, yolk, albumen, and embryo). The results showed measurable 14 C PCB 77 in all egg components, with changing concentrations in each egg component over the course of embryonic development. Specifically, concentrations in the shell content decreased between embryonic days 1 and 10, increased in albumen from embryonic days 1 to 5 and then decreased at embryonic day 10, and increased in both yolk and embryo from embryonic days 1 to 10. Vehicle and injection site both influenced 14 C PCB 77 allantoic fluid concentrations, with little effect on other egg components except for the inner shell membrane. The fatty acid vehicle injected into the albumen yielded the highest 14 C PCB 77 recovery. These findings demonstrate dynamic movement of toxicants throughout the egg components during avian embryonic development and a steady increase of relatively low levels of 14 C PCB 77 in the embryo compared with the yolk, albumen, and shell, suggesting that embryonic uptake (i.e., exposure) mirrors utilization of egg components for nutrition and growth during development. Environ Toxicol Chem 2018;37:126-135. © 2017 SETAC. |
| Databáze: | OpenAIRE |
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