Expression of bone morphogenetic protein 2 and fibroblast growth factor 2 during bone regeneration using different implant materials as an onlay bone graft in rabbit mandibles
| Autor: | Teruhisa Ohara, Kiyomasa Nakagawa, Daisuke Takazakura, Takashi Hase, Kohei Marukawa, Koichiro Ueki, Shamiul Alam |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Calcium Phosphates
Male Bone Regeneration Polymers Polyesters Basic fibroblast growth factor H&E stain Bone Morphogenetic Protein 2 Mandible Bone morphogenetic protein Bone morphogenetic protein 2 chemistry.chemical_compound Transforming Growth Factor beta Animals Medicine Lactic Acid Bone regeneration General Dentistry Bone Transplantation business.industry Regeneration (biology) Anatomy Recombinant Proteins Radius Otorhinolaryngology chemistry Bone Morphogenetic Proteins Bone Substitutes Models Animal Fibroblast Growth Factor 2 Surgery Rabbits Implant Oral Surgery business |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 103:16-26 |
| ISSN: | 1079-2104 |
| DOI: | 10.1016/j.tripleo.2006.01.019 |
| Popis: | 金沢大学医学部附属病院歯科口腔外科 Objectives: The purpose of this study was to histologically and immunohistochemically evaluate bone regeneration using 3 different implant materials in rabbit mandibles and to compare the bone regenerative capability of these materials in an animal model. Study design: Adult male Japanese white rabbits (n = 48; 12-16 wks old; 2.5-3.0 kg) were divided into 4 groups, consisting of 12 animals each. The implant materials were β-tricalcium phosphate (β-TCP), autologous bone derived from the radius, and recombinant human bone morphogenetic protein 2 (rhBMP-2) with polylactic acid/polyglycolic acid copolymer and gelatin sponge (PGS) complex. After incising along the inferior border of the mandible, the materials were implanted as only grafts and covered by titanium mesh with screws. No material was implanted into the control group. The rabbits were killed at 2, 4, 8, 12, and 24 wks postoperatively, and formalin-fixed specimens containing titanium mesh were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with BMP-2 and fibroblast growth factor 2 (FGF-2) antibodies. Finally, they were examined microscopically. Results: The autologous bone induced substantially more new bone formation compared with β-TCP at 4 wks postoperatively. However, rhBMP-2/PGS induced new bone formation at 8 wks postoperatively. No growth of bony tissue was observed in the control group at any period. In the autologous bone and rhBMP-2/PGS groups, both BMP-2 and FGF-2 were observed later in the β-TCP group than in other groups. Conclusion: This study suggests that autologous bone as well as rhBMP-2/PGS implants induce expression of both BMP-2 and FGF-2 specifically at the operated sites, even at early stages. © 2007 Mosby, Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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