Up-regulation of carcinoembryonic antigen-related cell adhesion molecule 1 in gastrointestinal cancer and its clinical relevance
| Autor: | Muqing Zhou, Lian Cui, Yingzhi Wang, Yiwen Liu, Yan Du, Manlin Cao, Jiajie Hu, Yiqing He, Zhiming Jin, Cuixia Yang, Feng Gao |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Short Communication Biophysics Enzyme-Linked Immunosorbent Assay medicine.disease_cause Biochemistry gastrointestinal neoplasms 03 medical and health sciences 0302 clinical medicine Carcinoembryonic antigen Antigen Antigens CD Medicine Humans Protein Isoforms Gastrointestinal cancer CEACAM1 Aged Aged 80 and over biology business.industry Cell adhesion molecule Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cancer General Medicine Middle Aged medicine.disease Immunohistochemistry ROC curve Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology Tumor progression 030220 oncology & carcinogenesis Cancer research biology.protein Female business Carcinogenesis Cell Adhesion Molecules |
| Zdroj: | Acta Biochimica et Biophysica Sinica |
| ISSN: | 1745-7270 1672-9145 |
| Popis: | Serum carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is dysregulated in various malignant tumors and has been associated with tumor progression. However, the expression and regulatory mechanisms of serum CEACAM1 in gastrointestinal cancer are still unclear. The expression ratio of the CEACAM1-L and CEACAM1-S isoforms has seldom been investigated in gastrointestinal cancer. In this study, we intended to explore the expression and diagnostic value of CEACAM1 in gastrointestinal cancer. Serum CEACAM1 levels were measured by enzyme-linked immunosorbent assay. The protein expression and distribution of CEACAM1 in tumors were examined by immunohistochemical staining. The expression patterns and ratio of CEACAM1-L/S were analyzed by reverse transcription-polymerase chain reaction. The results showed that serum CEACAM1 levels were significantly higher in cancer patients than in healthy controls. CEACAM1 was found in secreted forms within the neoplastic glands, and its expression was more intense at the tumor invasion front. The CEACAM1-L/S (L:S) ratios were up-regulated during tumorigenesis. Our data suggest that the serum level of CEACAM1 may be used to discriminate gastrointestinal cancer patients from health controls. |
| Databáze: | OpenAIRE |
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