The application of capillary microsampling in GLP toxicology studies
Autor: | Lieve Dillen, Hans Stieltjes, Philip Timmerman, Bianca Feyen, Loeckie de Zwart, Tom Verhaeghe, Ann Vroman, Luc Diels |
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Rok vydání: | 2017 |
Předmět: |
Drug-Related Side Effects and Adverse Reactions
Capillary action Clinical Biochemistry Drug Evaluation Preclinical Blood volume Pharmacology Volume sampling Toxicology 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry Toxicology studies 03 medical and health sciences 0302 clinical medicine Animals General Pharmacology Toxicology and Pharmaceutics Blood Specimen Collection Chromatography Chemistry 010401 analytical chemistry General Medicine 0104 chemical sciences Capillaries Rats Medical Laboratory Technology Pharmaceutical Preparations Laboratories |
Zdroj: | Bioanalysis. 9(7) |
ISSN: | 1757-6199 |
Popis: | Aim: Capillary microsampling (CMS) to collect microplasma volumes is gradually replacing traditional, larger volume sampling from rats in GLP toxicology studies. Methodology: About 32 µl of blood is collected with a capillary, processed to plasma and stored in a 10- or 4-µl capillary which is washed out further downstream in the laboratory. CMS has been standardized with respect to materials, assay validation experiments and application for sample analysis. Conclusion: The implementation of CMS has resulted in blood volume reductions in the rat from 300 to 32 µl per time point and the elimination of toxicokinetic satellite groups in the majority of the rat GLP toxicology studies. The technique has been successfully applied in 26 GLP studies for 12 different projects thus far. |
Databáze: | OpenAIRE |
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