Modulation of muscle protein synthesis by insulin is maintained during neonatal endotoxemia
| Autor: | Renán A. Orellana, M. Carole Thivierge, Teresa A. Davis, Jill A. Bush, Marta L. Fiorotto, Hanh V. Nguyen, Pamela M. J. O'Connor, Agus Suryawan |
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| Rok vydání: | 2006 |
| Předmět: |
Blood Glucose
Lipopolysaccharides medicine.medical_specialty Hydrocortisone Swine Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Protein metabolism Muscle Proteins Biology Sepsis Random Allocation chemistry.chemical_compound Insulin resistance Pregnancy Physiology (medical) Internal medicine medicine Protein biosynthesis Animals Insulin Muscle Skeletal Skeletal muscle Glucose clamp technique medicine.disease Endotoxemia Endocrinology medicine.anatomical_structure Animals Newborn chemistry Protein Biosynthesis Glucose Clamp Technique Female Amino Acids Branched-Chain medicine.drug |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 291:E159-E166 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.00595.2005 |
| Popis: | Sepsis promotes insulin resistance and reduces protein synthesis in skeletal muscle of adults. The effect of sepsis on insulin-stimulated muscle protein synthesis has not been determined in neonates, a highly anabolic population that is uniquely sensitive to insulin. Overnight fasted neonatal pigs were infused for 8 h with endotoxin [lipopolysaccharide (LPS), 0 and 10 μg·kg−1·h−1]. Glucose and amino acids were maintained at fasting levels, insulin was clamped at either fasting or fed (2 or 10 μU/ml) levels, and fractional protein synthesis rates were determined at the end of the infusion. LPS infusion induced a septic-like state, as indicated by a sustained elevation in body temperature, heart rate, and cortisol. At fasting insulin levels, LPS reduced fractional protein synthesis rates in gastrocnemius muscle (−26%) but had no effect on the masseter and heart. By contrast, LPS stimulated liver protein synthesis (+28%). Increasing insulin to fed levels accelerated protein synthesis rates in gastrocnemius (controls by +38%, LPS by +60%), masseter (controls by +50%, LPS by +43%), heart (controls by +34%, LPS by +40%), and diaphragm (controls by +54%, LPS by +29%), and the response to insulin was similar in LPS and controls. Insulin did not alter protein synthesis in liver, kidney, or jejunum in either group. These findings suggest that acute endotoxemia lowers basal fasting muscle protein synthesis in neonates but does not alter the response of protein synthesis to insulin. |
| Databáze: | OpenAIRE |
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