Recruitment of LC3 to damaged Golgi apparatus

Autor: Allan Sauvat, Wei Xie, Sylvie Souquere, Franck Perez, Ana Joaquina Jimenez, Guido Kroemer, Oliver Kepp, Séverine Divoux, Øystein Rekdal, Luis G. Arnaut, Lígia C. Gomes-da-Silva, Baldur Sveinbjørnsson, Marko Storch
Přispěvatelé: Institut Gustave Roussy (IGR), Fudan University, Fudan University [Shanghai], Rétrovirus endogènes et éléments rétroïdes des eucaryotes supérieurs (UMR 9196), Centre National de la Recherche Scientifique (CNRS)-Institut Gustave Roussy (IGR)-Université Paris-Sud - Paris 11 (UP11), Department of Life Sciences, Imperial College London, London, United Kingdom, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Département de Structure Subcellulaire et Dynamique Cellulaire [Paris], Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Rétrovirus endogènes et éléments rétroides des eucaryotes supérieurs (REERES (UMR 8122)), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Université Paris Dauphine-PSL-Institut Curie-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Cell Death and Differentiation
Cell Death and Differentiation, Nature Publishing Group, 2019, 26 (8), pp.1467-1484. ⟨10.1038/s41418-018-0221-5⟩
Cell Death Differ
ISSN: 1350-9047
1476-5403
DOI: 10.1038/s41418-018-0221-5⟩
Popis: LC3 is a protein that can associate with autophagosomes, autolysosomes, and phagosomes. Here, we show that LC3 can also redistribute toward the damaged Golgi apparatus where it clusters with SQSTM1/p62 and lysosomes. This organelle-specific relocation, which did not involve the generation of double-membraned autophagosomes, could be observed after Golgi damage was induced by various strategies, namely (i) laser-induced localized cellular damage, (ii) local expression of peroxidase and exposure to peroxide and diaminobenzidine, (iii) treatment with the Golgi-tropic photosensitizer redaporfin and light, (iv) or exposure to the Golgi-tropic anticancer peptidomimetic LTX-401. Mechanistic exploration led to the conclusion that both reactive oxygen species-dependent and -independent Golgi damage induces a similar phenotype that depended on ATG5 yet did not depend on phosphatidylinositol-3-kinase catalytic subunit type 3 and Beclin-1. Interestingly, knockout of ATG5 sensitized cells to Golgi damage-induced cell death, suggesting that the pathway culminating in the relocation of LC3 to the damaged Golgi may have a cytoprotective function.
Databáze: OpenAIRE
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