Clinical virology of Ebola hemorrhagic fever (EHF): virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995
| Autor: | Pierre E. Rollin, P. A. Leman, F. J. Burt, A. J. Williams, Clarence J. Peters, Mary Lane Martin, Ali S. Khan, Thomas G. Ksiazek, Rose Mukunu, Alexander K. Rowe, Anthony Sanchez, David S. Bressler, R. Swanepoel |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
viruses Enzyme-Linked Immunosorbent Assay Viral Plaque Assay medicine.disease_cause Antibodies Viral Virus Immunoglobulin G Disease Outbreaks Ebola Hemorrhagic Fever Virus antigen Antigen Virology Chlorocebus aethiops medicine Immunology and Allergy Animals Humans Antigens Viral Vero Cells Virus quantification Ebola virus biology Hemorrhagic Fever Ebola Ebolavirus Infectious Diseases Immunoglobulin M Immunology biology.protein Democratic Republic of the Congo Rabbits |
| Zdroj: | The Journal of infectious diseases. 179 |
| ISSN: | 0022-1899 |
| Popis: | Ebola hemorrhagic fever (EHF) patients treated at Kikwit General Hospital during the 1995 outbreak were tested for viral antigen, IgG and IgM antibody, and infectious virus. Viral antigen could be detected in virtually all patients during the acute phase of illness, while antibody was not always detectable before death. Virus was also isolated from patients during the course of their febrile illness, but attempts to quantify virus in Vero E6 cells by standard plaque assay were often unsuccessful. IgG and IgM antibody appeared at approximately the same time after disease onset (8-10 days), but IgM persisted for a much shorter period among the surviving convalescent patients. IgG antibody was detectable in surviving patients through about 2 years after onset, the latest time that samples were obtained. Detection of Ebola virus antigens or virus isolation appears to be the most reliable means of diagnosis for patients with suspected acute EHF, since patients with this often-fatal disease (80% mortality) may not develop detectable antibodies before death. |
| Databáze: | OpenAIRE |
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