Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media
| Autor: | Haruka Ishida, Hisao Haniu, Chuang Ma, Naoto Saito, Katsuya Ueda, Hirokazu Ideta, Takeshi Uemura, Atsushi Sobajima, Makoto Izumiya, Miyu Haniu, Koki Ueshiba |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
collagen vitamin C Biocompatible Materials 02 engineering and technology Ascorbic Acid calcification Mice Osteogenesis Biology (General) Spectroscopy Chemistry Biomaterial Osteoblast General Medicine 3T3 Cells 021001 nanoscience & nanotechnology Computer Science Applications Cell biology medicine.anatomical_structure osteoblast Alkaline phosphatase 0210 nano-technology alkaline phosphatase biomaterials QH301-705.5 Catalysis Collagen Type I Article Cell Line Inorganic Chemistry 03 medical and health sciences In vivo medicine Animals Physical and Theoretical Chemistry QD1-999 Molecular Biology Cell Proliferation cell culture Osteoblasts Cell growth Regeneration (biology) Organic Chemistry medicine.disease 030104 developmental biology Cell culture Culture Media Conditioned Biomarkers Calcification |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 7752, p 7752 (2021) Volume 22 Issue 14 |
| ISSN: | 1422-0067 |
| Popis: | Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials. Article International Journal of Molecular Sciences. 22(14):7752 (2021) |
| Databáze: | OpenAIRE |
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