Development and Validation of Monoclonal and Polyclonal Antibodies for the Detection of Immunoglobulin G of Bottlenose Dolphins (Tursiops Truncatus)
| Autor: | Diane G. Duke, Elliott R. Jacobson, Scott Gearhart, Linda G. Green, Michael Walsh, Hendrik H. Nollens, Beth Chittick, Paul A. Klein |
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| Rok vydání: | 2007 |
| Předmět: |
Male
0301 basic medicine 040301 veterinary sciences medicine.drug_class 030106 microbiology Monoclonal antibody Immunoglobulin G Serology 0403 veterinary science Erysipelothrix Infections Mice 03 medical and health sciences Antibody Specificity medicine Animals Seroconversion General Veterinary biology Antibodies Monoclonal Reproducibility of Results 04 agricultural and veterinary sciences Bottlenose dolphin biology.organism_classification Virology Bottle-Nosed Dolphin Polyclonal antibodies Monoclonal Immunology Erysipelothrix biology.protein Rabbits Antibody human activities |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 19:465-470 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063870701900502 |
| Popis: | Antibodies directed against species-specific immunoglobulin G (IgG) have a broad range of applications in serologic and immunologic research and in the development of clinical assays. Validated anti-IgG antibodies for marine mammal species are in short supply. The objective of this study was to produce and validate antibodies with specificity for IgG of the common bottlenose dolphin ( Tursiops truncatus). Bottlenose dolphin IgG was purified using protein G. Two mouse monoclonal antibodies and a rabbit polyclonal antibody were developed from mice and rabbits immunized with bottlenose dolphin IgG. The specificity of the monoclonal antibodies and the polyclonal antibody for bottlenose dolphin IgG was first verified by Western blot analysis and enzyme-linked immunosorbent assay (ELISA). For further validation, both monoclonal antibodies and the polyclonal antibody were incorporated in an indirect ELISA for the detection of the immune response of bottlenose dolphins to a vaccine antigen. Three bottlenose dolphins were immunized with a commercial Erysipelothrix rhusiopathiae vaccine, and serial blood samples were collected from all dolphins for measurement of levels of circulating antibodies. Seroconversion was observed in all 3 dolphins by use of both monoclonal antibodies and the polyclonal antibody. Circulating antibodies were detectable as early as 6 days after immunization in 1 dolphin. Peak antibody levels were detected 14 days after the immunization. The ability to detect seroconversion in all 3 immunized bottlenose dolphins firmly establishes the specificity of the monoclonal antibodies and the polyclonal antibody for IgG of the common bottlenose dolphin. |
| Databáze: | OpenAIRE |
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