Immunoelectrophoretic characterization of human mitochondrial aspartate aminotransferase purified by ion exchange and affinity chromatography
| Autor: | Ole Koldkjaer |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Tris
Chromatography Ion exchange Crossed immunoelectrophoresis Biochemistry (medical) Clinical Biochemistry Ion chromatography Mitochondria Liver General Medicine Chromatography Ion Exchange Biochemistry Chromatography Affinity Antibody production chemistry.chemical_compound chemistry Affinity chromatography Antigen Humans Aspartate Aminotransferases Mitochondrial Aspartate Aminotransferase Immunoelectrophoresis Two-Dimensional |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 122(3) |
| ISSN: | 0009-8981 |
| Popis: | Human mitochondrial aspartate aminotransferase (m-ASAT) was prepared for use as an antigen for antibody production and as a standard, i.e. a high degree of purity was demanded. The purification was performed by three ion exchange chromatography steps followed by affinity chromatography on aspartate coupled gel. Four preparations gave specific activities of 230 to 300 U/mg at 37°C. The purity of m-ASAT was assessed by crossed immunoelectrophoresis, which showed that the final preparation did not contain contaminating proteins. Immunization with the purified m-ASAT gave a good antibody response. |
| Databáze: | OpenAIRE |
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