Selective alterations in insulin receptor substrates-1, -2 and -4 in theca but not granulosa cells from polycystic ovaries
| Autor: | Magoffin Da, Hui-Wen Yen, Jakimiuk Aj, Munir I |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
endocrine system Embryology medicine.medical_specialty endocrine system diseases Insulin Receptor Substrate Proteins medicine.medical_treatment Biology Follicle Internal medicine Insulin receptor substrate Genetics medicine Animals Humans Insulin Ovarian follicle Molecular Biology Adaptor Proteins Signal Transducing Granulosa Cells urogenital system Intracellular Signaling Peptides and Proteins Obstetrics and Gynecology Cell Biology Phosphoproteins Polycystic ovary female genital diseases and pregnancy complications Receptor Insulin Insulin receptor medicine.anatomical_structure Endocrinology Reproductive Medicine Theca Theca Cells biology.protein Female Developmental Biology Polycystic Ovary Syndrome Signal Transduction |
| Zdroj: | Molecular human reproduction. 10(7) |
| ISSN: | 1360-9947 |
| Popis: | The elevated insulin concentrations that occur in many women with polycystic ovary syndrome (PCOS) can contribute significantly to ovarian hyperandrogenism. The objective of the present study was to compare the content of proximal insulin signalling molecules in theca and granulosa cells between polycystic ovaries and regular cycling controls. Individual follicles ð 3–7 mmÞ were obtained from 11 women with PCOS and 10 regularly cycling control women. The theca and granulosa cells were microdissected from each follicle. Total protein was extracted and signalling proteins were measured by western blot analysis. There was no difference in insulin receptor content between PCOS and controls in either theca or granulosa cells. Insulin receptor substrate (IRS)-1 and -2 were increased ð P< 0:05Þ; but IRS-4 was decreased ð P< 0:03Þ in PCOS theca cells. There were no changes in IRS-1, -2 or -4 in granulosa cells. IRS-3 was undetectable in all samples. There were no changes in phosphatidyl inositol-3 kinase catalytic subunits p110a or p110b in either theca or granulosa cells. These data demonstrate cell-specific alterations in IRS protein concentrations in theca cells from polycystic ovaries that are consistent with an exaggerated amplification of the insulin signal and which may play an important role in ovarian hyperandrogenism and thecal hyperplasia. |
| Databáze: | OpenAIRE |
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