Lifelong calorie restriction affects indicators of colonic health in aging C57Bl/6J mice
| Autor: | Stefano Salvioli, Wilma T. Steegenga, Gianfranco Picone, Dieuwertje E. Kok, Hauke Smidt, Benthe van der Lugt, Claudio Franceschi, Carolien Lute, Luca Laghi, Fenni Rusli, Michael Müller, Ellen Kampman, Jacques Vervoort |
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| Přispěvatelé: | Kok DEG, Rusli F, van der Lugt B, Lute C, Laghi L, Salvioli S, Picone G, Franceschi C, Smidt H, Vervoort J, Kampman E, Müller M, Steegenga WT |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Aging Nutrition and Disease Endocrinology Diabetes and Metabolism Metabolite Clinical Biochemistry Gut flora Biochemistry Tumours of the digestive tract Radboud Institute for Health Sciences [Radboudumc 14] chemistry.chemical_compound Mice Voeding Metabolisme en Genomica Microbiologie Lactobacillus Voeding en Ziekte Gene expression Metabolites Bifidobacterium Nutrition and Dietetics biology Metabolism and Genomics Phenotype Metabolisme en Genomica Nutrition Metabolism and Genomics medicine.medical_specialty Colon Calorie restriction Biochemie Gut microbiota Microbiology Bile Acids and Salts 03 medical and health sciences Immune system Voeding Internal medicine medicine Animals Molecular Biology Nutrition Caloric Restriction VLAG Inflammation Interleukin-6 Gene Expression Profiling Lipid metabolism biology.organism_classification Lipid Metabolism Diet Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Endocrinology chemistry Gene Expression Regulation Colonic health Energy Intake Energy Metabolism |
| Zdroj: | Journal of Nutritional Biochemistry, 56, 152-164 Journal of Nutritional Biochemistry 56 (2018) Journal of Nutritional Biochemistry, 56, pp. 152-164 |
| ISSN: | 0955-2863 |
| Popis: | Item does not contain fulltext Diminished colonic health is associated with various age-related pathologies. Calorie restriction (CR) is an effective strategy to increase healthy lifespan, although underlying mechanisms are not fully elucidated. Here, we report the effects of lifelong CR on indicators of colonic health in aging C57Bl/6J mice. Compared to an ad libitum control and moderate-fat diet, 30% energy reduction was associated with attenuated immune- and inflammation-related gene expression in the colon. Furthermore, expression of genes involved in lipid metabolism was higher upon CR, which may point towards efficient regulation of energy metabolism. The relative abundance of bacteria considered beneficial to colonic health, such as Bifidobacterium and Lactobacillus, increased in the mice exposed to CR for 28 months as compared to the other diet groups. We found lower plasma levels of interleukin-6 and lower levels of various metabolites, among which are bile acids, in the colonic luminal content of CR-exposed mice as compared to the other diet groups. Switching from CR to an ad libitum moderate-fat diet at old age (24 months) revealed remarkable phenotypic plasticity in terms of gene expression, microbiota composition and metabolite levels, although expression of a subset of genes remained CR-associated. This study demonstrated in a comprehensive way that CR affects indicators of colonic health in aging mice. Our findings provide unique leads for further studies that need to address optimal and feasible strategies for prolonged energy deprivation, which may contribute to healthy aging. |
| Databáze: | OpenAIRE |
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