Bio-functional hydrogel membranes loaded with chitosan nanoparticles for accelerated wound healing

Autor: Heather C. Aziz, Mudassir Abbasi, Zahid Hussain, Touba Khaliq, Muhammad Usman Minhas, Maryam Shafique, Muhammad Sohail, Arshad Mahmood, Syed Ahmed Shah, Mubeen Kousar, Shahzeb Khan
Rok vydání: 2021
Předmět:
Male
02 engineering and technology
Biochemistry
Polyvinyl alcohol
Rats
Sprague-Dawley
Chitosan
chemistry.chemical_compound
Structural Biology
Materials Testing
Hyaluronic acid
Hyaluronic Acid
Cefepime
Glucans
Drug Carriers
0303 health sciences
Biological Dressings
Chemistry
Hydrogels
General Medicine
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
Membrane
Pseudomonas aeruginosa
Female
0210 nano-technology
Porosity
medicine.drug
Staphylococcus aureus
Chemistry Techniques
Analytical
03 medical and health sciences
Oxygen permeability
Tensile Strength
Escherichia coli
medicine
Animals
Molecular Biology
030304 developmental biology
Wound Healing
technology
industry
and agriculture
Membranes
Artificial
Pullulan
Rats
Drug Liberation
Polyvinyl Alcohol
Microscopy
Electron
Scanning
Nanoparticles
Wound healing
Biomedical engineering
Zdroj: International Journal of Biological Macromolecules. 170:207-221
ISSN: 0141-8130
Popis: Wounds are often recalcitrant to traditional wound dressings and a bioactive and biodegradable wound dressing using hydrogel membranes can be a promising approach for wound healing applications. The present research aimed to design hydrogel membranes based on hyaluronic acid, pullulan and polyvinyl alcohol and loaded with chitosan based cefepime nanoparticles for potential use in cutaneous wound healing. The developed membranes were evaluated using dynamic light scattering, proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. The results indicated the novel crosslinking and thermal stability of the fabricated hydrogel membrane. The in vitro analysis demonstrates that the developed membrane has water vapors transmission rate (WVTR) between 2000 and 2500 g/m2/day and oxygen permeability between 7 and 14 mg/L, which lies in the range of an ideal dressing. The swelling capacity and surface porosity to liberate encapsulated drug (cefepime) in a sustained manner and 88% of drug release was observed. The cefepime loaded hydrogel membrane demonstrated a higher zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli and excisional rat model exhibit expeditious recovery rate. The developed hydrogel membrane loaded with cefepime nanoparticles is a promising approach for topical application and has greater potential for an accelerated wound healing process.
Databáze: OpenAIRE
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