Control of the cell-specificity of σ F activity in Bacillus subtilis
| Autor: | Jeff Errington, Yudkin, Andrea Feucht, Lord M, Wilkinson Jf, Peter J. Lewis, T Magnin, Seyed Mahmoud Arab Najafi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Transcription
Genetic Stereochemistry Molecular Sequence Data Phosphatase Sigma Factor Bacillus subtilis General Biochemistry Genetics and Molecular Biology Serine chemistry.chemical_compound Bacterial Proteins Transcription (biology) Sigma factor RNA polymerase Amino Acid Sequence Promoter Regions Genetic Spores Bacterial Sequence Homology Amino Acid biology Sigma DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial biology.organism_classification Molecular biology chemistry Genes Bacterial Phosphorylation General Agricultural and Biological Sciences Information Systems Transcription Factors |
| Zdroj: | Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences. 351:537-542 |
| ISSN: | 1471-2970 0962-8436 |
| DOI: | 10.1098/rstb.1996.0052 |
| Popis: | Sporulation in Bacillus subtilis is a simple developmental system involving the differentiation of two cell types that are formed by an asymmetric cell division. Major changes in the pattern of transcription during sporulation are brought about by the synthesis of new sigma factors (σ), which are subunits of RNA polymerase that determine promoter specificity. Transcription in the smaller prespore cell type is initiated by a sigma factor called σ F , the activity of which is subject to tight spatial and temporal control. It is negatively regulated by an anti-sigma factor, SpoIIAB, which is in turn controlled by an anti-anti-sigma factor, SpoIIAA. SpoIIAA and SpoIIAB participate in two contrasting reactions in vitro. In the presence of ATP, the proteins interact transiently and SpoIIAA is inactivated by phosphorylation on a specific serine residue; SpoIIAA then remains free to inhibit σ F . In the presence of ADP, SpoIIAA binds tightly to SpoIIAB, and σ F is set free. Release of σ F activity in vivo might thus be effected by a prespore-specific reduction in the ATP/A DP ratio. Genetic experiments have implicated a fourth protein, called SpoIIE, in this system. It now appears that SpoIIE has two important and independent functions in the establishment of the prespore-specific transcription by σ F . First it regulates σ F activity, probably acting as a phosphatase to regenerate the active, non-phosphorylated form of SpoIIAA. Second it controls the formation of the septum that generates the prespore compartment. Combination of these two functions in a single polypeptide may provide a means of coupling gene expression with morphogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|