Gold Nanobiosensor Based on the Localized Surface Plasmon Resonance is Able to Diagnose Human Brucellosis, Introducing a Rapid and Affordable Method
| Autor: | Ahmad Movahedpour, Mohammad Samare-Najaf, Reza Tabrizi, Saeed Taghizadeh, Amirreza Dehghanian, Ali Samareh, Mahdiyar Iravani Saadi, Amir Tajbakhsh, Hassan Askari, Saam Noroozi, Marzieh Alizadeh, Sina Vakili |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Nano Express biology LSPR Brucellosis Gold standard (test) Condensed Matter Physics biology.organism_classification medicine.disease Colloidal gold Direct agglutination test Diagnosis medicine TA401-492 Cutoff General Materials Science Surface plasmon resonance Nanobiosensor Materials of engineering and construction. Mechanics of materials Brucella melitensis Biomedical engineering Human brucellosis |
| Zdroj: | Nanoscale Research Letters, Vol 16, Iss 1, Pp 1-11 (2021) Nanoscale Research Letters |
| Popis: | Brucellosis is considered as the most common bacterial zoonosis in the world. Although the laboratory findings are the most reliable diagnosis today, the current laboratory methods have many limitations. This research aimed to design and evaluate the performance of a novel technique based on the localized surface plasmon resonance (LSPR) to eliminate or reduce existing shortcomings. For this purpose, smooth lipopolysaccharides were extracted from Brucella melitensis and Brucella abortus and fixed on the surface of the gold nanoparticles through covalent interactions. After some optimizing processes, dynamic light scattering was used to characterize the probe. The detection of captured anti-Brucella antibody was performed by measuring the redshift on LSPR peak followed by the determination of cutoff value, which indicated a significant difference between controls and true positive patients (P value |
| Databáze: | OpenAIRE |
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