Cold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to Repair Oxidised Membrane and Enhance Uptake of Nanomaterial in Glioblastoma Multiforme Cells

Autor: Brijesh K. Tiwari, Patrick J. Cullen, Kangze Liu, Furong Tian, Laurence Scally, Sing Wei Ng, Eline Manaloto, James F. Curtin, Hugh J. Byrne, Zhonglei He, Sebnem Gunes, Paula Bourke, Marcus A. Maher
Přispěvatelé: Science Foundation Ireland, 4/IA/2626, 16/BBSRC/3391
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Plasma Gases
Cytotoxicity
lcsh:Medicine
Metal Nanoparticles
glioblastoma multiforme cells
cold atmospheric plasma
01 natural sciences
Cell membrane
Lipid peroxidation
synergistic cytotoxicity
chemistry.chemical_compound
Cellular uptake
Nanotechnology
lcsh:Science
010302 applied physics
chemistry.chemical_classification
Multidisciplinary
Food Chemistry
Endocytosis
Food Biotechnology
medicine.anatomical_structure
Membrane
Cholesterol
Nanotechnology in cancer
nanomaterial
Article
03 medical and health sciences
Targeted therapies
clathrin
0103 physical sciences
Oxidation
medicine
Humans
Gold nanoparticles
Biochemistry
Biophysics
and Structural Biology
Reactive oxygen species
Neuronal degeneration
Toxicity
lcsh:R
Receptor-mediated endocytosis
Molecular mechanisms
Nanostructures
030104 developmental biology
Metabolism
chemistry
Cancer cell
cancer cells
Food Microbiology
Biophysics
lcsh:Q
(cAp)
nanoparticles
Gold
Glioblastoma
Reactive Oxygen Species
atmospheric plasma
Food Science
Zdroj: Scientific Reports
Articles
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Popis: Cold atmospheric plasma (CAP) enhances uptake and accumulation of nanoparticles and promotes synergistic cytotoxicity against cancer cells. However, the mechanisms are not well understood. In this study, we investigate the enhanced uptake of theranostic nanomaterials by CAP. Numerical modelling of the uptake of gold nanoparticle into U373MG Glioblastoma multiforme (GBM) cells predicts that CAP may introduce a new uptake route. We demonstrate that cell membrane repair pathways play the main role in this stimulated new uptake route, following non-toxic doses of dielectric barrier discharge CAP. CAP treatment induces cellular membrane damage, mainly via lipid peroxidation as a result of reactive oxygen species (ROS) generation. Membranes rich in peroxidised lipids are then trafficked into cells via membrane repairing endocytosis. We confirm that the enhanced uptake of nanomaterials is clathrin-dependent using chemical inhibitors and silencing of gene expression. Therefore, CAP-stimulated membrane repair increases endocytosis and accelerates the uptake of gold nanoparticles into U373MG cells after CAP treatment. We demonstrate the utility of CAP to model membrane oxidative damage in cells and characterise a previously unreported mechanism of membrane repair to trigger nanomaterial uptake. This knowledge will underpin the development of new delivery strategies for theranostic nanoparticles into cancer cells.
Databáze: OpenAIRE
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