Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR
| Autor: | Uwe Völker, Kristin Surmann, Andrea Ebert-Jung, Elke Hammer, Sebastian Wörner, Gottfried Unden |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Biology medicine.disease_cause Microbiology DNA-binding protein Mass Spectrometry 03 medical and health sciences Fumarates Transcriptional regulation medicine Escherichia coli Dicarboxylic Acids Anaerobiosis Phosphorylation Molecular Biology Transcription factor Dicarboxylic Acid Transporters Catabolism Kinase Escherichia coli Proteins Autophosphorylation Gene Expression Regulation Bacterial Aerobiosis DNA-Binding Proteins 030104 developmental biology Biochemistry Protein Kinases Signal Transduction Transcription Factors Research Article |
| Zdroj: | Journal of bacteriology. 200(4) |
| ISSN: | 1098-5530 |
| Popis: | In Escherichia coli , the catabolism of C 4 -dicarboxylates is regulated by the DcuS-DcuR two-component system. The functional state of the sensor kinase DcuS is controlled by C 4 -dicarboxylates (like fumarate) and complexation with the C 4 -dicarboxylate transporters DctA and DcuB, respectively. Free DcuS (DcuS F ) is known to be constantly active even in the absence of fumarate, whereas the DcuB-DcuS and DctA-DcuS complexes require fumarate for activation. To elucidate the impact of the transporters on the functional state of DcuS and the concentrations of DcuS F and DcuB-DcuS (or DctA-DcuS), the absolute levels of DcuS, DcuB, and DctA were determined in aerobically or anaerobically grown cells by mass spectrometry. DcuS was present at a constant very low level (10 to 20 molecules DcuS/cell), whereas the levels of DcuB and DctA were higher (minimum, 200 molecules/cell) and further increased with fumarate (12.7- and 2.7-fold, respectively). Relating DcuS and DcuB contents with the functional state of DcuS allowed an estimation of the proportions of DcuS in the free (DcuS F ) and the complexed (DcuB-DcuS) states. Unexpectedly, DcuS F levels were always low (F as the major species under noninducing conditions. In the absence of fumarate, when DcuS F is responsible for basal dcuB expression, up to 8% of the maximal DcuB levels are formed. These suffice for DcuB-DcuS complex formation and basal transport activity. In the presence of fumarate (>100 μM), the DcuB-DcuS complex drives the majority of dcuB expression and is thus responsible for induction. IMPORTANCE Two-component systems (TCS) are major devices for sensing by bacteria and adaptation to environmental cues. Membrane-bound sensor kinases of TCS often use accessory proteins of unknown function. The DcuS-DcuR TCS responds to C 4 -dicarboxylates and requires formation of the complex of DcuS with C 4 -dicarboxylate transporters DctA or DcuB. Free DcuS (DcuS F ) is constitutively active in autophosphorylation and was supposed to have a major role under specific conditions. Here, absolute concentrations of DcuS, DcuB, and DctA were determined under activating and nonactivating conditions by mass spectrometry. The relationship of their absolute contents to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo , which was not accessible by other approaches, leading to a revision of previous models. |
| Databáze: | OpenAIRE |
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