Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil
| Autor: | Maria Angélica Miglino, Janaína M. Monteiro, Hatawa Melo de Almeida, Osmar Ferreira da Silva Filho, Jacyara de Jesus Rosa Pereira Alves, Flávio Ribeiro Alves, Napoleão Martins Argôlo Neto, Andressa Rego da Rocha, Matheus Levi Tajra Feitosa, Carlos Eduardo Ambrósio, Anaemilia das Neves Diniz, Laecio da Silva Moura, Maria Acelina Martins de Carvalho, Yulla Klinger Pereira de Carvalho, Kassio Vieira Macedo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Homeobox protein NANOG Stromal cell RD1-811 Cellular differentiation Cell Culture Techniques Bone Marrow Cells Biology Proliferating Cell Nuclear Antigen medicine Animals Vimentin Progenitor cell Cell Proliferation MESODERMA Stem Cells Goats Mesenchymal stem cell Mesenchymal Stem Cells Cell Differentiation Anatomy Flow Cytometry Molecular biology medicine.anatomical_structure Cell culture Models Animal Female Surgery Bone marrow Stem cell Octamer Transcription Factor-3 Brazil |
| Zdroj: | Acta Cirurgica Brasileira, Volume: 29, Issue: 8, Pages: 478-484, Published: AUG 2014 Acta Cirurgica Brasileira, Vol 29, Iss 8, Pp 478-484 (2014) Acta Cirúrgica Brasileira v.29 n.8 2014 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | PURPOSE:To characterize bone marrow progenitors cells grown in vitro,using native goats from northeastern Brazil as animal model.METHODS:Ten northeastern Brazil native goats of both genders were used from the Piaui Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog).RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog.CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied. |
| Databáze: | OpenAIRE |
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