Antioxidant and Gene Protective Effects of Medicinal Mushrooms Inonotus obliquus (Pers.:Fr.) Pilat and Phellinus robustus (P. Karst.) Bourd. et Galz
| Autor: | Victor V. Shcherba, Nadezda Yu. Mitropolskaya, Victor T. Bilay, Nina A. Bisko |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pharmacology
Antioxidant biology Molecular mass medicine.medical_treatment biology.organism_classification Applied Microbiology and Biotechnology Melanin chemistry.chemical_compound Pigment Biochemistry chemistry visual_art Drug Discovery medicine visual_art.visual_art_medium biology.protein Inonotus obliquus Gene DNA Peroxidase |
| Zdroj: | International Journal of Medicinal Mushrooms. :388 |
| ISSN: | 1521-9437 |
| DOI: | 10.1615/intjmedmushrooms.v7.i3.370 |
| Popis: | Fungal melanin pigments were shown to display high antioxidant activity. Th is work was designed to isolate melanin pigments from Inonotus obliquus and Phellinus robustus to study their antioxidant and gene protective properties during metabolic activation of aromatic amines by the peroxidase pathway of oxidation. Investigation of the element composition of exoand endomelanins of Inonotus obliquus and Phellinus robustus showed that these species distinguish one from another insignifi cantly. However, it has been demonstrated that the content of carboxyl groups in endomelanins of both investigated species was two times higher in comparison with exomelanins. IR- spectra of exo- and endomelanins of I. obliquus and Ph. robustus were also similar. Th molecular weight of exo- and endomelanines from I. obliquus was 35–60 kDa, and from Ph. robustus 40–60 kDa. Th e obtained data indicate that the alkaline fraction of exo- and endomelanins of I. obliquus had a molecular mass of 55–60 kDa, Ph. robustus 65–70 kDa, the alcohol fraction of I. obliquus 25–30 kDa, and Ph. robustus 40–45 kDa. Th e melanins of both species also contained a small quantity of substances with the molecular mass of 100–120 kDa. Melanins isolated from both mushrooms and applied at a concentration above 20 µg/mL inhibited the reaction of peroxidase-mediated oxidation of o-dianisidine (DA). It was demonstrated that the ability of melanin isolated from Ph. robustus to inhibit peroxidase-mediated free radical oxidation of DA was considerably lower than that of the pigment from I. obliquus. Th e highest degrees of inhibition of peroxidase-mediated DA oxidation with melanins isolated from Ph. robustus (75 µg/mL) and I. obliquus (75 µg/mL) mushrooms were 75% and 80%, respectively. Our data showed that melanin isolated from I. obliquus inhibited peroxidase-mediated oxidation of DA more effi ciently than it prevented damage to DNA induced by oxidation products. In contrast, melanin from Ph. robustus applied at a lower concentration inhibited the formation of DNA/DNA crosslinks more readily than it prevented DA oxidation. Such properties of melanins can be used in developing new anticarcinoganic drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|